Avidin Antikörper (Biotin)

Details for Product anti-Avidin Antibody No. ABIN116961, Anbieter: Anmelden zum Anzeigen
Antigen
  • avidin
  • avd
  • LOC723980
  • AVD
Reaktivität
Huhn
32
26
10
9
8
Wirt
Kaninchen
43
35
2
Klonalität
Polyklonal
Konjugat
Dieser Avidin Antikörper ist konjugiert mit Biotin
12
9
9
4
4
4
4
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
70
69
58
10
9
8
8
5
4
4
3
2
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Avidin [Hen Egg White]
Spezifität Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Avidin [Hen Egg White].
Produktmerkmale Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Reinigung A multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below
Hintergrund The biological function of avidin is not known. It is a homotetrameric glycoprotein found in chicken egg white, and forms a strong non covalent specific complex with biotin (one molecule of biotin per subunit of avidin).Synonyms: AVD
Gen-ID 396260
NCBI Accession NP_990651
UniProt P02701
Forschungsgebiet Epitope Tags, Tags/Labels
Applikationshinweise Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 μg of Avidin [Hen Egg White] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat)(R1364HRP) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 8,000 to 1: 32,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 1.0 mL of deionized water (or equivalent).
Konzentration 10.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing. Do NOT add sodium azide! Dilute only prior to immediate use.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.
Allgemeine Veröffentlichungen Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).

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