His Tag Antikörper

Details zu Produkt Nr. ABIN116941, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
612
26
15
3
3
Wirt
Kaninchen
411
184
27
23
4
4
3
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
58
45
36
17
14
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
549
389
164
109
78
78
74
25
13
10
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide
Sequenz HHHHHH
Reinigung Affinity Chromatography.
Andere Bezeichnung 6xHistidine Epitope Tag
Substanzklasse Tag
Hintergrund Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.
Applikationshinweise Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. This antibody has been tested by ELISA and western blotting against both the immunizingpeptide and His-containing recombinant proteins. Although not tested, this antibody islikely functional for Immunoprecipitation and Immunocytochemistry. Recommended Dilutions: ELISA: 1/10,000-1/40,000. Western blot: 1/1,000-1/3,000. Immunohistochemistry: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
Bilder des Herstellers
Western Blotting (WB) image for anti-His Tag antibody (ABIN116941) Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins b...
Allgemeine Veröffentlichungen Yi, Ba, Zhang, Ho, Chen: "Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain." in: Journal of virology, Vol. 79, Issue 18, pp. 11638-46, 2005 (PubMed).

Haben Sie etwas anderes gesucht?