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Rekombinanter eGFP Antikörper

eGFP Reaktivität: Aequorea victoria ELISA, IP, ICC Wirt: Human Monoclonal G3 unconjugated Recombinant Antibody
Produktnummer ABIN1169226
  • Target Alle eGFP Produkte
    eGFP (Enhanced Green Fluorescent Protein (eGFP))
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 11
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    Aequorea victoria
    Wirt
    • 16
    • 5
    • 1
    • 1
    Human
    Klonalität
    • 17
    • 6
    Monoklonal
    Konjugat
    • 9
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser eGFP Antikörper ist unkonjugiert
    Applikation
    • 20
    • 13
    • 7
    • 3
    • 3
    • 2
    • 2
    • 1
    ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Spezifität
    Recognizes EGFP, Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP).
    Aufreinigung
    Purified from HEK 293 cell culture supernatant.
    Reinheit
    >95 % (SDS-PAGE)
    Immunogen
    Enhanced Green Fluorescent Proteins (EGFP).
    Klon
    G3
    Isotyp
    IgG2
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    anti-EGFP, monoclonal antibody (recombinant) (G3) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    In PBS containing 10 % glycerol and 0.02 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
    Haltbarkeit
    12 months
  • Moutel, Vielemeyer, Jin, Divoux, Benaroch, Perez: "Fully in vitro selection of recombinant antibodies." in: Biotechnology journal, Vol. 4, Issue 1, pp. 38-43, (2009) (PubMed).

  • Target
    eGFP (Enhanced Green Fluorescent Protein (eGFP))
    Andere Bezeichnung
    EGFP (eGFP Produkte)
    Hintergrund
    Anti-EGFP, monoclonal antibody (recombinant) (G3) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
    UniProt
    P42212
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