Golgin B1 (GOLGB1) Antikörper

Details zu Produkt Nr. ABIN1169059, Anbieter: Anmelden zum Anzeigen
Antigen
  • gcp
  • golim1
  • giantin
  • 4930428L02Rik
  • 628101
  • 6330407A06Rik
  • AU042952
  • C130074L01Rik
  • F730017E11Rik
  • Gm6840
  • mKIAA4151
  • GCP
  • GCP372
  • GOLIM1
  • golgin B1
  • golgin B1 S homeolog
  • golgi autoantigen, golgin subfamily b, macrogolgin 1
  • GOLGB1
  • golgb1.S
  • Golgb1
Reaktivität
Human, Maus
33
13
10
2
2
1
1
1
1
1
1
Wirt
Human
17
13
2
2
2
1
Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
3
1
1
1
1
1
Applikation
Immunocytochemistry (ICC)
22
10
8
8
7
5
3
3
2
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Human recombinant giantin.
Klon TA10
Isotyp IgG2
Spezifität Recognizes human and mouse giantin.
Kreuzreaktivität Human, Maus
Reinigung Purified from HEK 293 cell culture supernatant.
Reinheit >95 % (SDS-PAGE)
Andere Bezeichnung Giantin (GOLGB1 Antibody Abstract)
Hintergrund Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
UniProt Q14789
Applikationshinweise Optimal working dilution should be determined by the investigator.
Kommentare

anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
Haltbarkeit 12 months
Produkt verwendet in: Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, 2010 (PubMed).

Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, 2003 (PubMed).

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