IL1A Antikörper

Produktnummer ABIN116863

Produktdetails anti-IL1A Antikörper

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1a in the same formats using similar dilutions. The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples. Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form. The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly. The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell. This antibody is also useful for Neutralization of human and primate IL-1a activity in bioassays. It does not neutralize the biological activity IL-1b. It does not neutralize the biological activity of mouse, rat or rabbit IL-1a. It will neutralize primate IL-a. For Neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Aufreinigung: DEAE fractionation.

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli. Remarks: The MW of the recombinant IL-1a was 17,000. This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).

Anwendungsinformationen

Applikationshinweise: This IgG fraction antibody of anti-Human IL-1a has been tested for use in Neutralizations,ELISA, Immunohistochemistry, Flow Cytometry and Immunoblotting. It recognizes the17,000 MW mature IL-1a . Reactivity in other immunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRPGoat-anti-Rabbit IgG [H&L] and TMB as a substrate. A working dilution range of 1: 100 to1: 200 is suggested for this application to detect IL-1a from supernatants or lysates of 2 x10^6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC arestimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. This producthas been assayed by Immunohistochemistry. A dilution range of 1: 200 is suggested for thisimmunoassay. Either paraffin fixation or cryofixation can be used forImmunohistochemistry using a dilution of 1: 200 for staining of intracellular IL-1a . This

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated thawing and freezing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized product at 2-8 °C. Store stock solution or aliquots thereof at -20 °C.

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: Interleukin-1 alpha / IL-1A (IL1A Produkte)

Synonyme: IL-1A antikoerper, IL1 antikoerper, IL1-ALPHA antikoerper, IL1F1 antikoerper, IL-6 antikoerper, Il-1a antikoerper, IL-1 alpha antikoerper, IL-1alpha antikoerper, L1A antikoerper, interleukin 1 alpha antikoerper, interleukin 6 antikoerper, IL1A antikoerper, IL6 antikoerper, Il1a antikoerper

Hintergrund: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1

Gen-ID: 3552

NCBI Accession: NP_000566

UniProt: P01583

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints