Urease (URE) Antikörper
| Antigen |
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| Synonyme |
F12B7.10, F12B7_10, URE, UREASE |
| Klonalität |
Polyklonal |
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Wirt
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Applikation
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 Alternativen Enzyme Immunoassay (EIA), Immunpräzipitation (IP), Western Blot (WB)
Western Blot (WB) (28), ELISA (24), Immunpräzipitation (IP) (12), Enzyme Immunoassay (EIA) (10), Immunfluoreszenz (IF) (9), Immunhistochemie (IHC) (7), Dot Blot (Dot) (2), Durchflusszytometrie (FACS) (1), Immunodiffusion (ID) (1), Immunelektronenmikroskopie (EM) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1), Radioimmunoassay (RIA) (1)
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| Produktnummer |
ABIN116848 |
| Menge |
50 mg (10.0 mg/ml (by UV absorbance at 280...) (Varianten) |
| Preis |
310,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: Urea amidohydrolase
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Weitere Bezeichnung
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Urease
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Swiss-Prot
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P07374
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Immunogen
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Urease [Jack Bean].
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Spezifität
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This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-RabbitSerum as well as purified and partially purified Urease [Jack Bean]. Cross reactivity against Urease from other tissues and species may occur but have notbeen specifically determined
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Applikationshinweise
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suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry. Recommended Diutions: This product has been assayed against 1.0 ug of Urease [JackBean] in a standard capture ELISA using Peroxidase conjugated Affinity Purified anti-RabbitIgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at room temperature. A working dilution of 1: 15,000 to 1: 60,000of the reconstitution concentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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10.0 mg/ml (by UV absorbance at 280 nm)
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Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) sodium azide as a preservative.Reconstitution: Restore with 5.0 ml of deionized water (or equivalent).
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Lagerung
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Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and thenaliquot contents and freeze at -20°C or below. Centrifuge product if not completely clearafter standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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Forschungsgebiet
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Enzyme, Metabolismus
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Urease (URE)", Typ "Antikörper" finden
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Wirte
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Kaninchen (34)
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Reaktivitäten
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Schwertbohne (11), Jackbean (Canavalia ensiformis) (3), Pflanzen (2)
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Applikationen
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Western Blot (WB) (28), ELISA (24), Immunpräzipitation (IP) (12), Enzyme Immunoassay (EIA) (10), Immunfluoreszenz (IF) (9), Immunhistochemie (IHC) (7), Dot Blot (Dot) (2), Durchflusszytometrie (FACS) (1), Immunodiffusion (ID) (1), Immunelektronenmikroskopie (EM) (1), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (1), Radioimmunoassay (RIA) (1)
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Konjugate
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HRP (10), Biotin (9)
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