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GFP Antikörper (Biotin)

GFP Reaktivität: Aequorea victoria WB, IF, IHC (fro), EIA Wirt: Ziege Polyclonal Biotin
Produktnummer ABIN116746
  • Target Alle GFP Antikörper anzeigen
    GFP (Green Fluorescent Protein (GFP))
    Reaktivität
    • 180
    • 19
    • 15
    • 15
    • 12
    • 7
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Aequorea victoria
    Wirt
    • 76
    • 74
    • 35
    • 11
    • 9
    • 6
    • 5
    • 1
    Ziege
    Klonalität
    • 123
    • 89
    Polyklonal
    Konjugat
    • 106
    • 14
    • 12
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GFP Antikörper ist konjugiert mit Biotin
    Applikation
    • 173
    • 87
    • 56
    • 42
    • 34
    • 33
    • 32
    • 21
    • 16
    • 14
    • 14
    • 14
    • 12
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Spezifität
    This GFP antibody was prepared from monospecific antiserum by Immunoaffinity Chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
    Kreuzreaktivität (Details)
    Ads. to Hu, Ms, Rt Serum Proteins
    Produktmerkmale
    Molar Ratio: 10-20 BAC molecules per Goat IgG molecule
    Aufreinigung
    Immunoaffinity Chromatography
    Immunogen
    GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
    Top Product
    Discover our top product GFP Primärantikörper
  • Applikationshinweise
    Polyclonal anti-GFP antibody is designed to detect GFP and its variants. This antibody canbe used to detect GFP by ELISA (Sandwich or Capture) for the direct binding of antigen andrecognizes wild type, recombinant and enhanced forms of GFP. Biotin conjugated polyclonal anti-GFP antibody used in a Sandwich ELISA is well suited totitrate GFP in solution using this antibody in combination with a monoclonal anti-GFPantibody(Cat#R1461P) using either form of the antibody as the capture or detection
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1.0 mL of deionized water (or equivalent).
    Konzentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL BSA (IgG and Protease free) as stabilizer.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.
  • Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, (2012) (PubMed).

    Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, (2009) (PubMed).

  • Target
    GFP (Green Fluorescent Protein (GFP))
    Andere Bezeichnung
    GFP (GFP Produkte)
    Synonyme
    green fluorescent protein antikoerper, gfp antikoerper
    Substanzklasse
    Viral Protein
    Hintergrund
    Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
    Gen-ID
    6100
    UniProt
    P42212
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