Glucose Oxidase (GOD) Antikörper

Antigen: Glucose Oxidase (GOD)

Synonyme: LOC406081, GB19418

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Enzyme Immunoassay (EIA), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN116720

Produktbeschreibung

Produktmerkmale: Synonyms: Glucose oxyhydrase, Beta-D-glucose: oxygen 1-oxido-reductase, gox

Weitere Bezeichnung: Glucose Oxidase

Swiss-Prot: P13006

Immunogen: Glucose Oxidase [Aspergillus niger].

Beschreibung: Glucose Oxidase is widely used for the determination of glucose in body fluids and inremoving residual glucose or oxygen from foods and beverages. Glucose Oxidaseproducing moulds such as Aspergillus and Penicillium species are used for the biologicalproduction of gluconic acid. CATALYTIC ACTIVITY: Beta D glucose + O2 = D glucono 1,5 lactone + H2O2.

Spezifität: This product is an IgG fraction antibody purified from monospecific antiserum by amultistep process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified andpartially purified Glucose Oxidase and against anti-rabbit serum. Cross reactivity against Glucose Oxidase from other tissues and species may occur but hasnot been specifically determined.

Anwendungen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA and most Immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Glucose Oxidase[Aspergillus niger] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 30,000 of the reconstitution concentration is suggestedfor this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide as preservativeReconstitution: Restore with 5.0 ml of deinized water or equivalent.

Lagerung: Store the lyophilized antibody at 2-8°C. Following reconstitution store at 2-8°C for up toone month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Forschungsgebiet: Signaltransduktion, Metabolismus, Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar