Choline Oxidase Antikörper

Antigen: Choline Oxidase

Klonalität: Polyklonal

Wirt: Ziege

Applikation: Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN116684

Produktbeschreibung

Swiss-Prot: P16101

Immunogen: Choline Oxidase [Alcaligenes species].

Beschreibung: Choline Oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine withconcomitant consumption of oxygen and production of hydrogen peroxide.

Spezifität: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-GoatSerum as well as purified and partially purified Choline Oxidase [Alcaligenes species]. Cross reactivity against Choline Oxidase from other sources is unknown.

Anwendungen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Choline Oxidase[Alcaligenes species] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Goat IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 22,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 10.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%sodium azide as preservative.Reconstitution: Restore with 5.0 ml of deionized water (or equivalent).

Lagerung: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage aliquot contents and freeze at -20°Cor below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Forschungsgebiet: Signaltransduktion, Metabolismus, Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar