L-Ascorbate Oxidase Antikörper

Antigen: L-Ascorbate Oxidase

Synonyme: LOC100281551, LOC100282094, LOC100285297, LOC100285637

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Kürbis (Cucurbita)

Applikation: Enzyme Immunoassay (EIA), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN116624

Produktbeschreibung

Produktmerkmale: Synonyms: Ascorbase, ASO, AAO

Swiss-Prot: P24792

Immunogen: Ascorbate Oxidase [cucurbita].

Format: Serum

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified andpartially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidasefrom other tissues and species may occur but have not been specifically determined.

Anwendungen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: : This product has been assayed against 1.0 ug of AscorbateOxidase [cucurbita] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 6,000 to 1: 23,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 85.0 mg/ml (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%sodium azide as preservative.Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).

Lagerung: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage aliquot contents and freeze at -20°Cor below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Forschungsgebiet: Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar