RFX5 Antikörper (N-Term)

Produktnummer ABIN116534

Produktdetails anti-RFX5 Antikörper

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RFX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Spezifität: Anti-RFX5 (N-terminal specific) may react with unknown minor bands at 85 kDa and 55 kDa. Reacts with human and mouse RFX5 from a variety of fibroblast and B-cell lysates. Recognition is at the N-terminal region. Western blot of RFX5 shows a 75 kDa band. A minor band at 68 kDa may be detected which may represent an RFX5 breakdown product or unmodified RFX5.

Aufreinigung: Prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: RFX5 (N-terminal specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoprecipitation, Western blotting (1/1000), ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against RFX5 (N-terminal specific) from a variety of fibroblast and B-cell lysates ata dilution of 1: 500 followed by reaction with Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat). Anti-RFX5 (N-terminal specific) is suitable for the detection byimmunoblot of human RFX5 a shows a 75 kDa band. This product was also tested in a gelsupershift assay and found to be reactive against RFX5 complexes using 0.5 to 1.0 l perassay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative., None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.

Details zu RFX5

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Andere Bezeichnung: RFX5 (RFX5 Produkte)

Synonyme: regulatory factor X5 antikoerper, regulatory factor X, 5 (influences HLA class II expression) antikoerper, RFX5 antikoerper, Rfx5 antikoerper

Hintergrund: RFX5 is the regulatory gene responsible for human MHC class II deficiency. Patients with this disease exhibit a characteristic defect in the binding of a nuclear factor, RFX, to the X box motif of MHC class II promoters. RFX5 is the 75 kDa subunit of the RFX complex which is absolutely essential and highly specific for regulating MHC class II gene expression and control of the immune response. RFX5 is a protein consisting of 616 amino acids.Synonyms: DNA-binding protein RFX5, Regulatory factor X 5

Gen-ID: 5993

NCBI Accession: NP_000440

UniProt: P48382