This antibody may react non-specifically with other proteins. This antibody is suitable for the detection by immunoblot of Human, Mouse and Rat IκB-alpha. Control Peptide (R1013CP) will compete only with the specific reaction of antiserum with IκB-alpha.
Aufreinigung
Prepared from monospecific antiserum by Delipidation and Defibrination.
Immunogen
IkBa peptide corresponding to a region near the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
NFKBIA
Reaktivität: Human, Maus, Ratte
WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Suitable for Immunoblotting (1/2,000-1/10,000), ELISA (1/20,000-1/100,000) andImmunohistochemistry (1/1,000-1/5,000). Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IκBa showing a 36 kDa band. Perform all incubations except colordevelopment using TBS supplemented with 0.1 % Tween-20 at room temperature. Block themembrane with 5 % dry milk for 2 h. Add a 1: 1,000 dilution of the primary antibody to themembrane and incubated for 2 h. Perform washes with buffer 4 times for 5' each. Incubatethe immunoblot with Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] at1/2,000 for 1 h. Wash with TBS only preceded color development. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
80 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.09 % (w/v) Sodium Azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
NFKBIA
(Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
IKBA antikoerper, MAD-3 antikoerper, NFKBI antikoerper, AI462015 antikoerper, Nfkbi antikoerper, RL/IF-1 antikoerper, ECI-6/IKBA antikoerper, NFKBIA antikoerper, ikbaB antikoerper, wu:fb81f08 antikoerper, zgc:56666 antikoerper, zgc:77616 antikoerper, ikbaA antikoerper, wu:fj16a07 antikoerper, zgc:85769 antikoerper, NFKB inhibitor alpha antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b antikoerper, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a antikoerper, NFKBIA antikoerper, Nfkbia antikoerper, nfkbiab antikoerper, nfkbiaa antikoerper
Hintergrund
Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI