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CCL4 Antikörper

CCL4 Reaktivität: Human WB, IHC (p), EIA, Func Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN116168
  • Target Alle CCL4 Antikörper anzeigen
    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
    Reaktivität
    • 58
    • 41
    • 17
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 89
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    Kaninchen
    Klonalität
    • 93
    • 8
    Polyklonal
    Konjugat
    • 48
    • 17
    • 12
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CCL4 Antikörper ist unkonjugiert
    Applikation
    • 87
    • 48
    • 33
    • 15
    • 13
    • 13
    • 13
    • 13
    • 9
    • 6
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
    Spezifität
    Recognizes Human Macrophage Inflammatory Protein-1 beta (MIP-1b). Other species not tested.
    Aufreinigung
    Affinity Chromatography employing immobilized Human MIP-1beta matrix.
    Immunogen
    Highly pure (>98%) E.coli derived recombinant Human MIP-1 beta (Cat.-No PA107)
    Top Product
    Discover our top product CCL4 Primärantikörper
  • Applikationshinweise
    Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-1 beta (50.00 ng/mL), a concentration of 1.75-3.00 μg/mL of this antibody is required. ELISA: To detect hMIP-1 beta by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMIP-1 beta. Western Blot: To detect hMIP-1 beta by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-1 beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
    Buffer
    PBS, pH 7.2 without preservatives
    Konservierungsmittel
    Without preservative
    Handhabung
    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Schlaepfer, Audigé, Joller, Speck: "TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).

  • Target
    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
    Andere Bezeichnung
    MIP1 beta / CCL4 (CCL4 Produkte)
    Synonyme
    ACT2 antikoerper, AT744.1 antikoerper, G-26 antikoerper, HC21 antikoerper, LAG-1 antikoerper, LAG1 antikoerper, MIP-1-beta antikoerper, MIP1B antikoerper, MIP1B1 antikoerper, SCYA2 antikoerper, SCYA4 antikoerper, CCL4 antikoerper, K203 antikoerper, Mip1-b antikoerper, Scya4 antikoerper, MIP-1beta antikoerper, MIP-1BETA antikoerper, MIP1b antikoerper, SCY4A antikoerper, Act-2 antikoerper, MIP-1B antikoerper, Mip1b antikoerper, ACT-2 antikoerper, AT744.2 antikoerper, CCL4L antikoerper, SCYA4L antikoerper, SCYA4L1 antikoerper, SCYA4L2 antikoerper, C-C motif chemokine ligand 4 antikoerper, chemokine (C-C motif) ligand 4 antikoerper, chemokine (C-C motif) ligand 4-like 1 antikoerper, C-C motif chemokine ligand 4 like 1 antikoerper, CCL4 antikoerper, Ccl4 antikoerper, CCL4L1 antikoerper
    Hintergrund
    MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.Synonyms: CCL-4, G-26 T-lymphocyte-secreted protein, HC21, LAG1, Lymphocyte activation gene 1 protein, MIP-1-beta, MIP1B, Macrophage inflammatory protein 1-beta, PAT744, Protein H400, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2
    Gen-ID
    388372
    NCBI Accession
    NP_001001435
    UniProt
    P13236
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