CXCL1 Antikörper (Biotin)

Produktnummer ABIN115928

Produktdetails anti-CXCL1 Antikörper (Biotin)

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCL1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: Recognises Murine KC.

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mKC (murine KC).

Anwendungsinformationen

Applikationshinweise: ELISA: To detect mKC by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mKC. Sandwich: To detect mKC by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine KC (PP023P1 or PP023P2) as a captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mKC. Western Blot:

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Details zu CXCL1

Target: CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Andere Bezeichnung: CXCL1 / GRO-alpha (CXCL1 Produkte)

Synonyme: FSP antikoerper, GRO1 antikoerper, GROa antikoerper, MGSA antikoerper, MGSA-a antikoerper, NAP-3 antikoerper, SCYB1 antikoerper, CINC-1 antikoerper, Gro1 antikoerper, CXCL1 antikoerper, Sdf1 antikoerper, Fsp antikoerper, KC antikoerper, Mgsa antikoerper, N51 antikoerper, Scyb1 antikoerper, gro antikoerper, GRO antikoerper, Gro antikoerper, C-X-C motif chemokine ligand 1 antikoerper, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) antikoerper, C-X-C motif chemokine ligand 12 antikoerper, chemokine (C-X-C motif) ligand 1 antikoerper, CXCL1 antikoerper, Cxcl1 antikoerper, Cxcl12 antikoerper, GRO1 antikoerper

Hintergrund: The GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93 % and 82 % identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils.Synonyms: CXCL1, GRO, GRO-alpha(1-73), GRO1, GROA, Growth-regulated alpha protein, MGSA, Melanoma growth stimulatory activity, NAP-3, Neutrophil-activating protein 3, SCYB1

Gen-ID: 14825

NCBI Accession: NP_032202

UniProt: P12850

Pathways: Autophagie