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IL12A Antikörper

IL12A Reaktivität: Maus WB, EIA, Func Wirt: Ratte Monoclonal unconjugated
Produktnummer ABIN115777
  • Target Alle IL12A Antikörper anzeigen
    IL12A (Interleukin 12 alpha (IL12A))
    Reaktivität
    • 58
    • 12
    • 6
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 51
    • 14
    • 5
    • 2
    • 1
    Ratte
    Klonalität
    • 58
    • 15
    Monoklonal
    Konjugat
    • 42
    • 10
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser IL12A Antikörper ist unkonjugiert
    Applikation
    • 55
    • 31
    • 30
    • 9
    • 8
    • 7
    • 7
    • 6
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
    Spezifität
    Detects Mouse IL-12. Other species not tested.
    Aufreinigung
    Affinity Chromatography on Protein G
    Immunogen
    Recombinant murine IL-12
    Isotyp
    IgG1
    Top Product
    Discover our top product IL12A Primärantikörper
  • Applikationshinweise
    ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 3-4 μg/mL of thisantibody will detect at least 0.1 ng/well of recombinant murine IL-12 when used withbiotinylated antigen affinity purified anti-murine IL-12 as the detection antibody. Neutralisation: To yield one-half maximal inhibition (ND50) of the biological activity ofmurine IL-12 (2.0 ng/mL), a concentration of 0.16-0.24 μg/mL of this antibody is required. Western Blot: To detect mIL-12 by Western Blot analysis this antibody can be used at aconcentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-12 is 1.0-2.0 ng/lane, under reducing conditionsand 0.25-0.50 ng/lane, under non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore in sterile water to a concentration of 1.0 mg/mL.
    Buffer
    PBS without preservatives
    Konservierungsmittel
    Without preservative
    Handhabung
    Avoid repeated freezing and thawing. Protect from light!
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody at 2-8 °C under subdued light for one month or (in aliquots) at -20 °C for longer.
  • Target
    IL12A (Interleukin 12 alpha (IL12A))
    Andere Bezeichnung
    Interleukin-12 alpha / IL12A (IL12A Produkte)
    Synonyme
    CLMF antikoerper, IL-12A antikoerper, NFSK antikoerper, NKSF1 antikoerper, P35 antikoerper, CLMF2 antikoerper, IL-12B antikoerper, NKSF antikoerper, NKSF2 antikoerper, p40 antikoerper, Il-12b antikoerper, Il12p40 antikoerper, Il-12p40 antikoerper, Il12 antikoerper, IL-12p40 antikoerper, IL-12p35 antikoerper, Il-12a antikoerper, Ll12a antikoerper, p35 antikoerper, IL12p35 antikoerper, Il-12 p35 antikoerper, IL12p40 antikoerper, IL12 antikoerper, IL-12 p35 antikoerper, interleukin 12A antikoerper, interleukin 12B antikoerper, interleukin 12b antikoerper, interleukin 12a antikoerper, Interleukin 12 level antikoerper, IL12A antikoerper, IL12B antikoerper, Il12b antikoerper, Il12a antikoerper, IL12 antikoerper, il12a antikoerper
    Hintergrund
    Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1
    Gen-ID
    16159
    NCBI Accession
    NP_032377
    UniProt
    P43431
    Pathways
    JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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