KI-67 Antikörper (Antigen Identified By Monoclonal Antikörper Ki-67) (C-Term)

Details for Product anti-MKI67 Antibody No. ABIN115709, Anbieter: Anmelden zum Anzeigen
Antigen
  • MKI67
  • kia
  • mki67
  • MGC132156
  • D630048A14Rik
  • Ki-67
  • Ki67
  • KIA
  • MIB-1
  • antigen identified by monoclonal antibody Ki-67
  • antigen identified by monoclonal antibody Ki 67
  • MKI67
  • mki67
  • Mki67
Epitop
C-Term
92
54
49
18
18
15
15
14
8
6
5
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Ratte (Rattus)
457
113
71
17
16
9
4
2
1
Wirt
Kaninchen
260
158
32
9
5
3
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser KI-67 Antikörper ist unkonjugiert
17
13
11
10
10
8
7
6
5
4
4
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
187
183
158
120
119
105
85
73
16
13
13
11
6
3
3
3
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen A synthetic peptide from C-terminus of Human Ki-67.
Klon SP6
Isotyp IgG
Spezifität Reacts with Ki-67.
Cellular Localization: Nuclear.Cellular Localization:
Kreuzreaktivität (Details) Species reactivity (tested):Human and Rat.
Reinigung Supernatant
Andere Bezeichnung Ki-67 (MKI67 Antibody Abstract)
Hintergrund Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.Synonyms: Ki67 antigen, MKI67, Proliferation marker
Gen-ID 4288
NCBI Accession NP_001139438
UniProt P46013
Forschungsgebiet Proliferation Markers, Cell Cycle
Pathways Glycosaminoglycan Metabolic Process
Applikationshinweise Immunohistochemistry on Paraffin Sections: Use Ki67 antibody at a 1/100 dilution in anABC method for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil. Also suitable for Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) (C-Term) antibody (ABIN115709) Formalin-fixed, paraffin-embedded human tonsil stained with Ki-67 using peroxidase-co...
Produkt verwendet in: Goyvaerts, Lemaire, Arijs, Auffret, Granvik, Van Lommel, Binart, int Veld, Schuit, Schraenen: "Prolactin receptors and placental lactogen drive male mouse pancreatic islets to pregnancy-related mRNA changes." in: PLoS ONE, Vol. 10, Issue 3, pp. e0121868, 2015 (PubMed).

Jeong, Jung, Son, Jang, Lee, Kim, Ko, Lee, Lee: "Coniferyl aldehyde attenuates radiation enteropathy by inhibiting cell death and promoting endothelial cell function." in: PLoS ONE, Vol. 10, Issue 6, pp. e0128552, 2015 (PubMed).

Lee, Son, Jang, Bae, Kim, Kang, Kim: "Protective Effect of Administered Rolipram against Radiation-Induced Testicular Injury in Mice." in: The world journal of men's health, Vol. 33, Issue 1, pp. 20-9, 2015 (PubMed).

Yoon, Seo, Lee, Moon, Lee: "Acute high-level toluene exposure decreases hippocampal neurogenesis in rats." in: Toxicology and industrial health, 2015 (PubMed).

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