ELISA. Western Blot. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 0.05 mL of deionized water
Konzentration
1.0 mg/mL
Buffer
0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE
Konservierungsmittel
Azide free
Handhabung
Avoid repeated freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 months.
Haltbarkeit
6 months
Hara, Ikeda, Shimada, Nagamine, Sawa, Oda: "[Surgical experience of 6 multiple primary lung cancers]" in: Kyobu geka. The Japanese journal of thoracic surgery, Vol. 61, Issue 3, pp. 183-7, (2008) (PubMed).
Midkine (MK, also called neurite growth promoting factor 2, NEGF-2), a product of a retinoic acid responsive gene, is a secreted 13 kDa protein belonging to the family of heparin binding growth/differentiation factors. MK shares 45 % sequence identity with other member of this family called Pleiotrophin (HB-GAM). Midkine is composed of two domains held together by disulfide linkages. The C-terminally located domain contains two heparin binding sites and is usually responsible for midkine activity. Part of the MK activity is enhanced by dimerization of MK. Midkine has been found in vertebrates from human to zebrafish and is most strongly expresed in midgestation. In the adult MK expression is restricted. In addition to normal development, MK is also involved in the pathogenesis of diseases e.g. inflammatory diseases, human carcinomas such as esophageal, stomach, colon, pancreatic, thyroid, lung, urinary, hepatocellular, neuroblastoma, glioblastoma, Wilm´s tumor etc. High MK levels are associated with poor prognosis in some type of cancer. The increased expresion in many carcinomas indicates that MK can be applied to the diagnosis of malignancy. Midkine is expressed during the reparative stage of bone fractures, also supresses infection of certain viruses including HIV in target cells. Anti-apoptotic and cell protecting activity of midkine makes it to be a promissing in therapy.Synonyms: Amphiregulin-associated protein, MDK, MK, MK1, Midgestation and kidney protein, NEGF2, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein