Adenovirus Type 2 + 5 E1A Antikörper

Antigen: Adenovirus Type 2 + 5 E1A

Klonalität: Monoklonal (M58-M73)

Wirt: Maus

Applikation: Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN115166

Produktbeschreibung

Produktmerkmale: Synonyms: Adenovirus antibody

Immunogen: BALB/C mice were injected with adenovirus.

Format: Supernatant

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: M58-M73

Beschreibung: Adenoviruses are DNA viruses generally widespread in nature that are frequently the causeof acute upper respiratory tract infections (i. e. common colds). Forty-seven knownserotypes have been isolated since they were first discovered in 1953 with 3 types knownto cause gastroenteritis. Several types have oncogenic potential though most causeself-limiting febrile illnesses characterised by inflammation of conjunctivae and therespiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgicallyremoved, indicating latent infections. It is not known how long the virus can persist in thebody, or whether it is capable of reactivation after long periods. In patients experiencingimmunosuppression (e. g. AIDS) it can be reactivated causing disease. The adenovirus early gene products E1A is a potent stimulator of cellular proliferation,which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1Aregion encodes a series of related proteins (35-46 kD) with multifunctional capabilities andforms a specific complex with the retinoblastoma tumor suppressor gene product. The E1aand E1B regions together comprise the transforming region of adenovirus. Whileexpression of E1A alone is sufficient to immortalize primary cells, complete transformationrequires the additional expression of the E1B region.

Spezifität: This antibody reacts with adenovirus. Cellular Localization: NuclearSpecies: Human. Others not tested.

Anwendungen

Applikationshinweise: Immunohistochemistry on paraffin embedded sections: diluted of 1: 50-1: 75 in an ABC method (30 minutes at room temperature)Recommended Positive Control: Adenovirus infected tissueOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: 10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Virologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Formalin fixed paraffin embedded human infected appendix stained with Adenovirus antibody. ABIN115165

Publikationen

Publikationen: Harlow, Whyte, Franza et al.: "Association of adenovirus early-region 1A proteins with cellular polypeptides." in: Molecular and cellular biology, Vol. 6, Issue 5, pp. 1579-89, 1987 (PubMed).

Klein: "The approaching era of the tumor suppressor genes." in: Science (New York, N.Y.), Vol. 238, Issue 4833, pp. 1539-45, 1988 (PubMed).