Keratin 19 (KRT19) Antikörper

Details zu Produkt Nr. ABIN114797, Anbieter: Anmelden zum Anzeigen
Antigen
  • CK19
  • K19
  • K1CS
  • AI663979
  • EndoC
  • Krt-1.19
  • Krt1-19
  • Ka19
  • k19
  • ck19
  • k1cs
  • krt9
  • krt15
  • MGC76282
  • GK-19
  • MGC83069
  • KRT19
  • keratin 19
  • keratin 19 L homeolog
  • keratin, type I cytoskeletal 19
  • KRT19
  • Krt19
  • krt19
  • krt19.L
  • LOC100344434
  • LOC101117946
Reaktivität
Human
910
211
136
19
18
15
11
9
7
5
5
3
3
3
2
2
1
1
1
1
1
1
1
Wirt
Maus
681
223
24
5
2
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
52
32
30
20
17
17
17
17
17
16
16
16
15
15
15
15
12
8
7
6
4
3
1
1
1
Applikation
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
630
475
329
314
305
292
232
190
107
19
15
12
11
10
3
3
2
2
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Human breast cancer cell line MCF-7
Klon Ks19-1 (A53-B-A2)
Isotyp IgG2a
Spezifität Specific for the rod domain (aa 312-335) of Human Cytokeratin 19. In Western blotting 40 kDa and 19 kDa bands are observed.
Kreuzreaktivität (Details) Species reactivity (tested):Human.
Reinigung Affinity Chromatography on Protein G.
Andere Bezeichnung Cytokeratin 19 (KRT19 Antibody Abstract)
Hintergrund This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19
Gen-ID 3880
NCBI Accession NP_002267
UniProt P08727
Forschungsgebiet Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Applikationshinweise ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 1/100-1/200 (Requires protein digestionpre-treatment of paraffin sections e. g. trypsin or pronase). Use appendix as Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Keratin 19 (KRT19) antibody (ABIN114797) Staining of paraffin embedded human appendix with mouse anti human cytokeratin 19 (AB...
Allgemeine Veröffentlichungen Goletz, Hanisch, Karsten: "Novel alphaGalNAc containing glycans on cytokeratins are recognized invitro by galectins with type II carbohydrate recognition domains." in: Journal of cell science, Vol. 110 ( Pt 14), pp. 1585-96, 1997 (PubMed).

Kasper, Stosiek, Typlt, Karsten: "Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues." in: European journal of cancer & clinical oncology, Vol. 23, Issue 2, pp. 137-47, 1988 (PubMed).

Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, 1985 (PubMed).