Prostate Specific Antigen Antikörper

Produktnummer ABIN2853579

Produktdetails anti-Prostate Specific Antigen Antikörper

Target: Prostate Specific Antigen (PSA)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Prostate Specific Antigen Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), ELISA (Capture)

Spezifität: DM1035 reacts with PSA. This antibody is reactive to normal prostate, benign prostate hyperplasia and prostate malignant carcinoma. It has not reacted with other normal tissues tested.

Kreuzreaktivität (Details): Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Human Prostate Specific Antigen

Klon: CHYH1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: In sandwich ELISA this antibody can be used as a Capture antibody in combinationwith a Monoclonal tracer/detection antibody (HRP conjugate, TMB as substrate). Recommended Capture Coating Dose: 0.5-1.0 μg/well. Under the suggested conditionsapproximately 1 ng/mL of PSA in human serum can be detected with an assay range of0-200 ng/mL. Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections (ABC method). Western blot: 10 μg/mL allows visualization of 1 μg/lane human PSA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with double distilled water to a final concentration of 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Andere Bezeichnung: Kallikrein-3 / PSA / KLK3 (PSA Produkte)

Synonyme: APS antikoerper, KLK2A1 antikoerper, PSA antikoerper, hK3 antikoerper, KLK3 antikoerper, Klk1c10l2 antikoerper, RSGK-50 antikoerper, RSKG-50 antikoerper, rGK-3 antikoerper, Kal-3 antikoerper, Kal3 antikoerper, Klk3 antikoerper, AAP-S antikoerper, MP100 antikoerper, Psa antikoerper, R74825 antikoerper, goku antikoerper, kallikrein related peptidase 3 antikoerper, kallikrein 3 antikoerper, kallikrein 1-related peptidase C3 antikoerper, kallikrein B, plasma 1 antikoerper, aminopeptidase puromycin sensitive antikoerper, KLK3 antikoerper, Klk1c3 antikoerper, Klkb1 antikoerper, Npepps antikoerper

Hintergrund: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors.Synonyms: Gamma-seminoprotein, P-30 antigen, Prostate-specific antigen, Semenogelase

Gen-ID: 354

NCBI Accession: NP_001025218

UniProt: P07288

Pathways: Komplementsystem