CD44 Antikörper (CD44) (Biotin)

Details for Product anti-CD44 Antibody No. ABIN114362, Anbieter: Anmelden zum Anzeigen
Antigen
  • AU023126
  • AW121933
  • AW146109
  • HERMES
  • Ly-24
  • Pgp-1
  • CD44A
  • METAA
  • RHAMM
  • CDW44
  • CSPG8
  • ECMR-III
  • HCELL
  • HUTCH-I
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU3
  • MIC4
  • Pgp1
  • CD44 antigen
  • CD44 antigen-like
  • Cd44 molecule
  • CD44 molecule (Indian blood group)
  • syndecan 4
  • Cd44
  • LOC100525150
  • CD44
  • SDC4
Reaktivität
Ratte (Rattus)
1232
458
225
126
80
63
56
51
47
46
38
37
31
30
28
11
9
4
3
3
2
2
2
2
2
2
1
1
1
1
1
Wirt
Maus
1071
301
228
16
3
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD44 Antikörper ist konjugiert mit Biotin
189
123
93
48
38
37
33
31
30
25
21
20
20
20
20
17
16
15
15
13
12
8
7
7
7
5
5
4
4
4
4
4
4
4
2
2
2
2
2
2
2
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1172
644
532
340
312
308
278
275
204
54
40
22
18
15
14
11
9
8
7
7
6
5
5
5
4
2
2
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen T cell blasts. Donor: BALB/c spleen. Fusion Partner: myeloma cell line NSO/1.
Klon OX-49
Isotyp IgG2a
Spezifität This monoclonal antibody recognizes rat CD44 (Pgp-1), also called CD44H. This antigen is expressed on most leukocytes (except a sub population of B cells) and increases upon activation. The OX-49 antibody binds extracellularly to the standard (S) form on rat leukocytes but it is not known if they bind to the N-terminal region. It has also been reported that the antibody may bind to melanoma cell lines that express CD44V (splice variant form).
Reinigung Protein G Chromatography
Andere Bezeichnung CD44 (CD44 Antibody Abstract)
Hintergrund CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
Gen-ID 10116
UniProt P26051
Forschungsgebiet Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle
Pathways Glycosaminoglycan Metabolic Process, Autophagie, Negative Regulation of intrinsic apoptotic Signaling
Applikationshinweise Flow Cytometry. Western Blotting. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1. 0-0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at 1: 500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: WistarCell Concentration: 1 x 10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: Biotin Mouse IgG2aCell Source Percentage of cells stained above control:
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD44 (CD44) antibody (Biotin) (ABIN114362) Mouse anti CD44 (HCAM) OX-49
Allgemeine Veröffentlichungen Wang, Zhan, Xu, Feuerstein, Wang: "Use of suppression subtractive hybridization for differential gene expression in stroke: discovery of CD44 gene expression and localization in permanent focal stroke in rats." in: Stroke; a journal of cerebral circulation, Vol. 32, Issue 4, pp. 1020-7, 2001 (PubMed).

Lewington, Padanilam, Martin, Hammerman: "Expression of CD44 in kidney after acute ischemic injury in rats." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 278, Issue 1, pp. R247-54, 2000 (PubMed).

Foster, Arkonac, Sibinga, Shi, Perrella, Haber: "Regulation of CD44 gene expression by the proinflammatory cytokine interleukin-1beta in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20341-6, 1998 (PubMed).

Arch, Wirth, Hofmann, Ponta, Matzku, Herrlich, Zöller: "Participation in normal immune responses of a metastasis-inducing splice variant of CD44." in: Science (New York, N.Y.), Vol. 257, Issue 5070, pp. 682-5, 1992 (PubMed).

Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, 1988 (PubMed).

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