T Cell Receptor (TCR) Vb 12 Antikörper

Antigen: T Cell Receptor (TCR) Vb 12

Klonalität: Monoklonal (CTVB12b)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN114355

Produktbeschreibung

Produktmerkmale: Synonyms: TCR V beta-12, TCR Vb12

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: CTVB12b

Spezifität: This monoclonal antibody reacts with TCR Vb12b bearing cells. Species: Mouse. Others not tested.

Anwendungen

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium (CL5030). 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x106 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of CL7212AP. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody CLCC40001 (FITC Goat anti-rat IgG (H+L)) at 1: 500dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are

Applikationshinweise: Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.2 mg/ml

Buffer: PBS and 0.09% NaN3

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch

Forschungsgebiet: Immunologie, Rezeptoren, Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar