MHC Class I H-2Dd Antikörper (FITC)

Details zu Produkt Nr. ABIN114288, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Maus
21
Wirt
Maus
21
Klonalität (Klon)
Monoklonal ()
Konjugat
FITC
4
3
2
Applikation
Flow Cytometry (FACS)
17
10
9
3
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Recipient: C3H/HeJ Immunocyte Donor: B6 x DBA/2 spleen cells Fusion Partner: SP2/0.Ag14
Klon 34-5-8S
Isotyp IgG2a
Spezifität This anti-mouse H-2Dd monoclonal antibody is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate cells bearing the H-2Dd(H-2.4) antigen from the appropriate strains of mice.
Reinigung Protein G Chromatography
Andere Bezeichnung MHC Class I H-2 Dd
Hintergrund Synonyms: D-D alpha chain, H-2 class I histocompatibility antigen, H-2D(D), H2-D1
Gen-ID 100045864
NCBI Accession XP_003086970
UniProt P01900
Applikationshinweise Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll FLOW CYTOMETRY ANALYSIS:
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-MHC Class I H-2Dd antibody (FITC) (ABIN114288) FIGURE1: LFL1 - Cell Source: Spleen - Percentage of Cells Stained Above Control: 97.9%
Allgemeine Veröffentlichungen Ozato, Mayer, Sachs: "Monoclonal antibodies to mouse major histocompatibility complex antigens." in: Transplantation, Vol. 34, Issue 3, pp. 113-20, 1982 (PubMed).

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