MHC Class I H-2Dd (0) Antikörper

Antigen: MHC Class I H-2Dd (0)

Klonalität: Monoklonal (34-5-8S)

Wirt: Maus

Reaktivität: Maus

Applikation: Zytotoxizitätstest (CyTox)

Produktnummer: ABIN114285

Produktbeschreibung

Produktmerkmale: Synonyms: H2-D1, H-2D(D), H-2 class I histocompatibility antigen, D-D alpha chain

Weitere Bezeichnung: MHC Class I H-2 Dd

Swiss-Prot: P01900

Immunogen: Recipient: C3H/HeJ. Donor: B6 x DBA/2 spleen cells. Fusion Partner: SP2/0. Ag14.

Format: Ascites

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 34-5-8S

Spezifität: This monoclonal antibody is specific for cells expressing the H-2D antigen coded for by thed haplotype. The reaction pattern of this antibody with a panel of inbred and recombinanthaplotypes demonstrates that the antibody detects a private determinant (H-2. m4) of theH-2Dd antigen. This antibody can be used to quantitate or eliminate cells bearing H-2Dd(H-2. 4) antigen from the appropriate strains of mice. This clone has been reported to workin blocking experiments. Species: Mouse. Others not tested.

Anwendungen

Protokoll: RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF H-2Dd BEARINGLYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium1 orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-M density cell separation medium. After washing, adjust thecell concentration to 1x10e7 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 25 and mix. Alternatively, pellet the cellsand resuspend in antibody diluted 1: 25 in Cytotoxicity Medium. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Cedarlane Low-Tox®-M Rabbit Complement, diluted

Konzentration: 1x10e6 cells per mlComplement: Low-Tox®-M Rabbit ComplementComplement 1: 10Procedure: Two stage cytotoxicity as described in, Recommended Method for DeterminingPercent of H-2Dd Bearing Cells in a Population.RESULTS - STRAIN DISTRIBUTION BY CYTOTOXICITY A

Lagerung: Prior to and following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Cytotoxic Index

Publikationen

Publikationen: Ozato, Mayer, Sachs: "Monoclonal antibodies to mouse major histocompatibility complex antigens." in: Transplantation, Vol. 34, Issue 3, pp. 113-20, 1982 (PubMed).