Ly6g Antikörper

Produktnummer ABIN114273

Produktdetails anti-Ly6g Antikörper

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Ly6g Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Cytotoxicity Test (CyTox)

Aufreinigung: Protein G Chromatography.

Klon: RB6-8C5

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: This antibody is suitable for studies of myeloid differentiation stages and their regulationsby cytokines. Applications include Flow Cytometry (1,2,3) complement-mediated depletion(4), Western blot staining (5) and both Frozen and Paraffin Sections (6).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 1-0. 2 µg* of ABIN114273. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody FITC Goat anti-rat IgG (H+L) . 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results-Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 1 µg/10e6 cellsIsotypic Control: Rat IgG2bCell Source-Percentage of cells stained above control: Thymus: 2. 0%Whole Blood Monocytes: 79. 1%Bone Marrow Macrophages: 87. 5%N. B. Appropriate control samples should always be included in any labelling studies. * For optimal results in various applications, it is recommended that each investigatordetermine dilutions appropriate for individual use. Strain Distribution by Flow Cytometry Analysis: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 1 µg/10e6 cellsStrains Tested: BALB/c, C57BL/6, CBA, C3H/He, AKR

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS and 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Ly6g

Target: Ly6g (Lymphocyte Antigen 6 Complex, Locus G (Ly6g))

Andere Bezeichnung: Ly6G / GR1 Neutrophil Marker (Ly6g Produkte)

Synonyme: Gr-1 antikoerper, Gr1 antikoerper, Ly-6G antikoerper, lymphocyte antigen 6 complex, locus G antikoerper, Ly6g antikoerper

Hintergrund: Synonyms: Gr-1 Granulocyte marker

Gen-ID: 10090

UniProt: P35461