You are viewing an incomplete version of our website. Please click to reload the website as full version.

CD90 Antikörper (THY1) (PE)

Details für Produkt anti-THY1 Antikörper Nr. ABIN114255, Anbieter: Anmelden zum Anzeigen
Antigen
  • CD90
  • CD7
  • T25
  • Thy-1
  • Thy-1.2
  • Thy1.1
  • Thy1.2
  • Thy-1 cell surface antigen
  • thymus cell antigen 1, theta
  • THY1
  • Thy1
Reaktivität
Maus
360
337
150
37
35
33
28
28
26
26
16
6
4
4
2
2
1
Wirt
Maus
424
202
100
18
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD90 Antikörper ist konjugiert mit PE
83
74
59
32
14
14
11
11
9
9
8
7
7
7
7
7
7
5
4
4
4
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
539
222
165
123
120
84
73
72
36
31
16
14
9
9
9
8
8
6
5
3
2
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Immunogen CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
Klon 5a-8
Isotyp IgG2b
Spezifität This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
Reinigung Protein G Chromatography.
Andere Bezeichnung CD90 (THY1 Antibody Abstract)
Hintergrund CD90 (Thy1) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
Gen-ID 21838
NCBI Accession NP_033408
UniProt P01831
Forschungsgebiet CD Antigens, Surface Receptors of Immune Cells, Experimental Models
Applikationshinweise Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5 µg* of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsIsotypic Control: PE-Mouse IgG2b,kCell Source Percentage of cells stained above control: Thymus: 98. 4%Spleen: 28. 8%Results - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 5 µg/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR/J
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung This product is photosensitive and should be protected from light
Lagerung 4 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD90 Antikörper (THY1)  (PE) (ABIN114255) Cell Source: Thymus - Percentage of cells stained above control: 98.4%
Allgemeine Veröffentlichungen Krieg, Gourley, Steinberg: "Association of murine lupus and thymic full-length endogenous retroviral expression maps to a bone marrow stem cell." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 9, pp. 3002-5, 1991 (PubMed).

Miyajima, Hirano, Hirose, Karasuyama, Okumura, Ovary: "Suppression by IL-2 of IgE production by B cells stimulated by IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 2, pp. 457-62, 1991 (PubMed).

Kruger, Riley: "The age-dependent loss of bone marrow B cell precursors in autoimmune NZ mice results from decreased mitotic activity, but not from inherent stromal cell defects." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 1, pp. 103-10, 1990 (PubMed).

Fine, Silverstone, Gasiewicz: "Impairment of prothymocyte activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 4, pp. 1169-76, 1990 (PubMed).