CD90 Antikörper (THY1) (FITC)

Details for Product anti-THY1 Antibody No. ABIN114252, Anbieter: Anmelden zum Anzeigen
Antigen
  • CD90
  • CD7
  • T25
  • Thy-1
  • Thy-1.2
  • Thy1.1
  • Thy1.2
  • Thy-1 cell surface antigen
  • thymus cell antigen 1, theta
  • THY1
  • Thy1
Alternativen
anti-Maus CD90 Antikörper für Activity Assay
Reaktivität
Maus
377
339
140
32
32
31
28
28
26
25
15
6
4
4
3
1
Wirt
Maus
422
203
106
18
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD90 Antikörper ist konjugiert mit FITC
76
71
57
32
18
14
14
13
12
10
8
8
8
8
8
8
8
6
5
5
5
5
5
4
4
4
3
3
2
2
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
529
229
168
135
112
98
88
84
35
33
16
13
10
9
8
8
6
5
3
2
2
1
1
Optionen
Hersteller
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Immunogen CBA/J. Donor: AKR/J Spleen. Fusion Partner: Spleen from immunized recipient fused with myeloma P3-NSI-1-Ag4-1.
Klon 5a-8
Isotyp IgG2b
Spezifität This monoclonal antibody reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (e.g. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype [e.g. AKR/J, B6.PL(74NS)].
Kreuzreaktivität (Details) Species reactivity (tested):Mouse.
Reinigung Protein G Chromatography.
Andere Bezeichnung CD90 (THY1 Antibody Abstract)
Hintergrund CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
Gen-ID 21838
NCBI Accession NP_033408
UniProt P01831
Forschungsgebiet CD Antigens, Surface Receptors of Immune Cells, Experimental Models
Pathways Cell-Cell Junction Organization
Applikationshinweise Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 1/50 -1/100 dilution in 50 µl of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive)7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1/50 in 50 µl/10e6 cellsIsotypic Control: FITC Mouse IgG2bCell Source Percentage of cells stained above control: Thymus: 97. 4%Spleen: 52. 6%Results - Strain Distribution: Tissue: SpleenCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1/100 in 50 µl/10e6 cellsStrains Tested: C57BL/6, C3H/He, CBA/J, BALB/c, ATL, AKR/JPositive: C57BL/6, C3H/He, CBA/J, BALB/c, ATLNegative: AKR
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD90 Antikörper (THY1)  (FITC) (ABIN114252) Cell Source: Thymus - Percentage of cells stained above control: 97.4%
Allgemeine Veröffentlichungen Krieg, Gourley, Steinberg: "Association of murine lupus and thymic full-length endogenous retroviral expression maps to a bone marrow stem cell." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 9, pp. 3002-5, 1991 (PubMed).

Haba, Nisonoff: "Induction of tolerance to syngeneic IgE in neonatal mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 3, pp. 807-11, 1991 (PubMed).

Kruger, Riley: "The age-dependent loss of bone marrow B cell precursors in autoimmune NZ mice results from decreased mitotic activity, but not from inherent stromal cell defects." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 1, pp. 103-10, 1990 (PubMed).

Fine, Silverstone, Gasiewicz: "Impairment of prothymocyte activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 4, pp. 1169-76, 1990 (PubMed).