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CD90 Antikörper (THY1) (Biotin)

Details für Produkt anti-THY1 Antikörper Nr. ABIN114251, Anbieter: Anmelden zum Anzeigen
Antigen
  • CD7
  • CD90
  • T25
  • Thy-1
  • Thy-1.2
  • Thy1.1
  • Thy1.2
Reaktivität
Maus
358
342
145
42
40
29
26
24
22
21
10
5
4
4
2
2
1
Wirt
Maus
422
204
98
17
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD90 Antikörper ist konjugiert mit Biotin
82
74
58
32
14
14
11
11
9
9
8
7
7
7
7
7
7
5
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
548
213
172
151
114
83
75
71
36
32
25
13
9
9
8
6
5
3
2
2
1
1
Hersteller
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Immunogen CBA/J Donor: AKR/J Spleen
Klon 5a-8
Isotyp IgG2b
Spezifität This antibody detects CD90 (Thy 1.2). It reacts with all T lymphocytes from mouse strains expressing the Thy 1.2 phenotype (i.e. C57BL/6, C3H/He, DBA/2, CBA/J, BALB/c), but does not react with lymphocytes expressing the Thy 1.1 phenotype (i.e. AKR/J, B6.PL (74 NS).
Kreuzreaktivität (Details) Species reactivity (tested):Mouse.
Reinigung Protein G Chromatography.
Andere Bezeichnung CD90 (THY1 Antibody Abstract)
Hintergrund CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein
Gen-ID 21838
NCBI Accession NP_033408
UniProt P01831
Forschungsgebiet CD Antigens, Surface Receptors of Immune Cells, Experimental Models
Applikationshinweise Flow Cytometry (protocol see below). Appropriate control samples should always be included in any labelling studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 2-0. 5 µg of this antibody per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1/500 dilution. 9. Incubate tubes at 4°C for 30-60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: CBA/JCell Concentration : 1x10e6 cells per testsAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: Biotin Mouse IgG2b,kCell Source: Percentage of cells stained above control: Thymus: 97. 8%Spleen: 35. 4%
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store the antibody undiluted (in aliquots) at -20 °C.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD90 Antikörper (THY1)  (Biotin) (ABIN114251) Mouse anti CD90 (Thy-1.2) 5a-8
Allgemeine Veröffentlichungen Krieg, Gourley, Steinberg: "Association of murine lupus and thymic full-length endogenous retroviral expression maps to a bone marrow stem cell." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 9, pp. 3002-5, 1991 (PubMed).

Haba, Nisonoff: "Induction of tolerance to syngeneic IgE in neonatal mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 3, pp. 807-11, 1991 (PubMed).

Miyajima, Hirano, Hirose, Karasuyama, Okumura, Ovary: "Suppression by IL-2 of IgE production by B cells stimulated by IL-4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 146, Issue 2, pp. 457-62, 1991 (PubMed).

Kruger, Riley: "The age-dependent loss of bone marrow B cell precursors in autoimmune NZ mice results from decreased mitotic activity, but not from inherent stromal cell defects." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 1, pp. 103-10, 1990 (PubMed).

Fine, Silverstone, Gasiewicz: "Impairment of prothymocyte activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 4, pp. 1169-76, 1990 (PubMed).