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L-Selectin Antikörper (FITC)

SELL Reaktivität: Maus FACS Wirt: Ratte Monoclonal MEL-14 FITC
Produktnummer ABIN114234
  • Target Alle L-Selectin (SELL) Antikörper anzeigen
    L-Selectin (SELL) (Selectin L (SELL))
    Reaktivität
    • 99
    • 76
    • 41
    • 29
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Maus
    Wirt
    • 96
    • 67
    • 49
    Ratte
    Klonalität
    • 159
    • 48
    • 3
    Monoklonal
    Konjugat
    • 73
    • 28
    • 23
    • 16
    • 13
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser L-Selectin Antikörper ist konjugiert mit FITC
    Applikation
    • 157
    • 56
    • 56
    • 53
    • 35
    • 31
    • 17
    • 13
    • 12
    • 10
    • 8
    • 7
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Spezifität
    This monoclonal antibody reacts with a 90 kDa protein which is involved with the homing of lymphocytes to peripheral lymph nodes.
    Aufreinigung
    Protein G Chromatography
    Immunogen
    Mouse B cell Lymphoma, 38C-14 Donor: Fischer Rat Spleen Fusion Partner: P3 X 63Ag8.653
    Klon
    MEL-14
    Isotyp
    IgG2a
    Top Product
    Discover our top product SELL Primärantikörper
  • Applikationshinweise
    Flow cytometry. Immunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2 - 0. 5 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most flurochromes are light sensitive). 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution by: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 2 µg/10e6 cellsIsotypic Control: FITC Rat IgG2aCell Source: Percentage of cells stained above control: Thymus: 88. 8%Spleen: 36. 6%Lymph Node: 74. 4%Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 2 µg/10e6 cells
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.1 mg/mL
    Buffer
    PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    L-Selectin (SELL) (Selectin L (SELL))
    Andere Bezeichnung
    CD62L / L-Selectin (SELL Produkte)
    Synonyme
    CD62L antikoerper, LAM1 antikoerper, LECAM1 antikoerper, LEU8 antikoerper, LNHR antikoerper, LSEL antikoerper, LYAM1 antikoerper, PLNHR antikoerper, TQ1 antikoerper, A.11 antikoerper, L-selectin antikoerper, LECAM-1 antikoerper, SELE antikoerper, AI528707 antikoerper, Lnhr antikoerper, Ly-22 antikoerper, Ly-m22 antikoerper, Lyam-1 antikoerper, Lyam1 antikoerper, selectin L antikoerper, selectin, lymphocyte antikoerper, SELL antikoerper, Sell antikoerper
    Substanzklasse
    Chemical
    Hintergrund
    L-selectin is expressed on most T and B lymphocytes, neutrophils, monocytes, eosinophils.1 Pre-incubation of lymphocytes with this antibody completely and specifically blocks binding of lymphocytes to high endothelial venules (HEV) in vitro 2,3,6 and the migration of lymphocytes to lymph nodes in vivo.2,3 Polymorphonuclear cells preincubated with this antibody do not migrate to the inflammatory foci.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL
    Gen-ID
    20343
    NCBI Accession
    NP_035476
    UniProt
    P18337
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