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ITGA4 Antikörper

ITGA4 Reaktivität: Maus FACS, IP, IHC (fro), Func Wirt: Ratte Monoclonal R1-2 unconjugated
Produktnummer ABIN114228
  • Target Alle ITGA4 Antikörper anzeigen
    ITGA4 (Integrin alpha 4 (ITGA4))
    Reaktivität
    • 140
    • 103
    • 36
    • 19
    • 14
    • 10
    • 9
    • 8
    • 8
    • 7
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 81
    • 56
    • 47
    Ratte
    Klonalität
    • 116
    • 70
    • 1
    Monoklonal
    Konjugat
    • 83
    • 18
    • 17
    • 14
    • 7
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ITGA4 Antikörper ist unkonjugiert
    Applikation
    • 112
    • 62
    • 43
    • 40
    • 31
    • 25
    • 12
    • 10
    • 10
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
    Spezifität
    This antibody reacts with alpha 4 integrin.
    Aufreinigung
    Protein G affinity purified immunoglobulin fraction
    Immunogen
    Peyers Patch HEV binding lymphoma line (TK1)
    Klon
    R1-2
    Isotyp
    IgG2b
    Top Product
    Discover our top product ITGA4 Primärantikörper
  • Applikationshinweise
    Flow cytometry (see protocol). Immunoprecipitation. Immunohistochemistry on frozen sections. Functional assays.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protokoll
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of ABIN114228. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at a 1/500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    PBS buffer with 0.02 % sodium azide as preservative
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    ITGA4 (Integrin alpha 4 (ITGA4))
    Andere Bezeichnung
    CD49d / ITGA4 (ITGA4 Produkte)
    Synonyme
    CD49D antikoerper, IA4 antikoerper, LOC100219853 antikoerper, integrin subunit alpha 4 antikoerper, integrin alpha 4 antikoerper, integrin subunit alpha 4 S homeolog antikoerper, ITGA4 antikoerper, Itga4 antikoerper, itga4.S antikoerper
    Hintergrund
    Alpha 4 integrin, which helps to mediate cell-cell and cell-matrix interactions. It combines with beta 1 and beta 7integrin to form VLA-4 and LPAM-1 (Peyers patch homing receptor) respectively. VLA-4 is expressed on most peripheral lymphocytes, thymocytes and monocytes. LPAM-1 is found on peripheral lymphocytes, but few thymocytes. Fibronectin and VCAM-1 act as ligands for both VLA-4 and LPAM-1. LPAM-1 also binds the mucosal vascular addressin MAdCAM-1. (1)Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
    Gen-ID
    16401
    NCBI Accession
    NP_034706
    UniProt
    Q00651
    Pathways
    Integrin Complex
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