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CD44 Antikörper (CD44) (Biotin)

Details für Produkt anti-CD44 Antikörper Nr. ABIN114206, Anbieter: Anmelden zum Anzeigen
Antigen
  • AU023126
  • AW121933
  • AW146109
  • CD44A
  • CDW44
  • CSPG8
  • ECMR-III
  • HCELL
  • HERMES
  • HUTCH-I
  • IN
  • LHR
  • Ly-24
  • MC56
  • MDU2
  • MDU3
  • METAA
  • MIC4
  • Pgp-1
  • Pgp1
  • RHAMM
Reaktivität
Maus
1093
411
186
89
54
54
46
46
40
38
34
32
30
26
22
7
3
3
3
2
1
1
1
1
Wirt
Ratte
964
288
188
16
2
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD44 Antikörper ist konjugiert mit Biotin
196
118
92
42
27
26
25
21
19
19
16
16
16
16
16
16
12
9
9
8
7
7
7
5
5
4
4
4
4
4
4
4
3
2
2
2
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1017
613
418
359
311
303
236
205
132
50
21
18
18
16
14
8
7
7
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6
5
5
5
2
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Bone Marrow Derived Stromal cells (clone BMS2). Donor: Lou/MN Rat. Fusion Partner: SP2/0.
Klon KM81
Isotyp IgG2a
Spezifität This monoclonal antibody recognizes a 95 kDa glycoprotein found on most hematopoietic cells (1). It is thought to be important in the regulation of migratory properties of lymphocytes during development and the regulation of the interaction with bone marrow stromal cells during hematopoiesis (2,3). CD44 functions as a receptor for hyaluronate, although some cells expressing CD44 do not bind hyaluronate (3,4). This antibody has been shown to inhibit the growth of lymphoid and myeloid cells in long term bone marrow cultures (3). It also blocks the adhesive interactions of B cell hybridomas to a cloned stromal line or to hyaluronate coated dishes (4).
Reinigung Protein G Chromatography.
Andere Bezeichnung CD44 (CD44 Antibody Abstract)
Hintergrund CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.Synonyms: CDw44, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein 1
Gen-ID 12505
NCBI Accession NP_001034240
UniProt P15379
Forschungsgebiet Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells, Cancer, Cell Cycle
Applikationshinweise Flow Cytometry. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue DistributionMouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2aResults - Strain Distribution: Cell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsStrains Tested: BALB/c, CBA/J, C3H/He, C57BL/6, SWRPositive: BALB/c, CBA/J, C3H/He, C57BL/6, SWR
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.1 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
 image for anti-CD44 Antikörper (CD44)  (Biotin) (ABIN114206) Rat anti CD44 (HCAM) (Ly-24, Pgp-1) KM81
Allgemeine Veröffentlichungen Miyake, Medina, Hayashi, Ono, Hamaoka, Kincade: "Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures." in: The Journal of experimental medicine, Vol. 171, Issue 2, pp. 477-88, 1990 (PubMed).

Miyake, Underhill, Lesley, Kincade: "Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 69-75, 1990 (PubMed).

Picker, De los Toyos, Telen, Haynes, Butcher: "Monoclonal antibodies against the CD44 [In(Lu)-related p80], and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 6, pp. 2046-51, 1989 (PubMed).

Lynch, Ceredig: "Ly-24 (Pgp-1) expression by thymocytes and peripheral T cells." in: Immunology today, Vol. 9, Issue 1, pp. 7-10, 1989 (PubMed).