CD3 (CD3E) Antikörper

Antigen: CD3 (CD3E)

Synonyme: T3E, TCRE, FLJ18683

Klonalität: Monoklonal (500-A2)

Wirt: Hamster

Reaktivität: Maus

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Immunpräzipitation (IP)

Produktnummer: ABIN114142

Produktbeschreibung

Produktmerkmale: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Weitere Bezeichnung: CD3

Swiss-Prot: P22646

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: 500-A2

Beschreibung: In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complexand is involved with signal transduction.

Spezifität: This anti-mouse CD3ε monoclonal antibody is specific for the εchain of mouse CD31. Immobolized 500A2 mAb can be used to stimulate mouse T cells in vitro1. A combination ofimmobilized 500A2 mAb and anti-mouse CD28 mAb is commonly used for the activation ofmouse T cells3. Species: Mouse. Others not tested.

Anwendungen

Protokoll: FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).

Applikationshinweise: Flow cytometry (see Protocols). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.2 mg/ml

Buffer: PBS, 0.09% NaN3

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Immunologie, Adaptive Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Havran, Allison: "Developmentally ordered appearance of thymocytes expressing different T-cell antigen receptors." in: Nature, Vol. 335, Issue 6189, pp. 443-5, 1988 (PubMed).

Havran, Grell, Duwe et al.: "Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 11, pp. 4185-9, 1989 (PubMed).