CD3 (CD3E) Antikörper (Biotin)

Antigen: CD3 (CD3E)

Synonyme: T3E, TCRE, FLJ18683

Klonalität: Monoklonal (500-A2)

Wirt: Hamster

Reaktivität: Maus

Konjugat: Biotin

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN114139

Produktbeschreibung

Produktmerkmale: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Weitere Bezeichnung: CD3

Swiss-Prot: P22646

Isotyp: IgG

Klon: 500-A2

Beschreibung: In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complexand is involved with signal transduction.

Spezifität: This anti-mouse CD3ε monoclonal antibody is specific for the ε chain of mouse CD31. Whenimmobilized this antibody can be used to stimulate mouse T cells in vitro1. A combinationof this antibody and anti-mouse CD28 mAb is commonly used for the activation of mouse Tcells. Species: Mouse. Others not tested.

Anwendungen

Protokoll: FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 1. 0 µg* of this AB per 1x10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-PE) at a 1: 20 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. MEDIAA. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Hamster IgG

Applikationshinweise: Flow Cytometry (see Protocols). (Reportet to be useful in immunoprecipitation, immunofluorescent staining andimmuno-histochemistry on acetone fixed frozen sections. )Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.1 mg/ml

Buffer: PBS, 0.1% NaN3 and EIA grade BSA as stabilizing protein to bring the totalprotein concentration to 4-5 mg/mlLabel: Biotin

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Immunologie, Adaptive Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Cell Source: Spleen Percentage of cells stained above control: 44.1%

Publikationen

Publikationen: Harding, McArthur, Gross et al.: "CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones." in: Nature, Vol. 356, Issue 6370, pp. 607-9, 1992 (PubMed).

Havran, Allison: "Developmentally ordered appearance of thymocytes expressing different T-cell antigen receptors." in: Nature, Vol. 335, Issue 6189, pp. 443-5, 1988 (PubMed).

Havran, Grell, Duwe et al.: "Limited diversity of T-cell receptor gamma-chain expression of murine Thy-1+ dendritic epidermal cells revealed by V gamma 3-specific monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 11, pp. 4185-9, 1989 (PubMed).

Havran, Poenie, Kimura et al.: "Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes." in: Nature, Vol. 330, Issue 6144, pp. 170-3, 1987 (PubMed).