ACTC1 Antikörper

Produktnummer ABIN112213

Produktdetails anti-ACTC1 Antikörper

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Reaktivität: Human, Kaninchen, Huhn, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ACTC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is specific for Fetal (Cardiac) isoform of Actin. Clone Ac1 represents an excellent marker for cardiac tissue. It discriminates fetal (cardiac) alpha-Actin from all other Actin isoforms. Mab Ac1-20.4.2 shows no cross reaction with other Actin isoforms present in skeletal and smooth muscle, provided that stringent conditions have been applied (see Special Treatment).

Kreuzreaktivität (Details): Species reactivity (tested):Human, Bovine, Rabbit and Chicken.

Aufreinigung: Affinity Chromatographyon Protein G.

Immunogen: Synthetic NH2 terminus decapeptide of cardiac isoform of Actin.

Klon: AC1-20-4-2

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1/500. Western Blot: 1/1000 (using the ECL-enhanced procedure). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 (After microwave treatment). Incubation time: 1 h at RT. Special Treatment: It is necessary to include 0.5 M NaCl in all washing buffers forapplication on Frozen tissue and 1 M NaCl for Western blotting and ELISA to enhancespecifity. This is not necessary for Paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1.0 mL distilled water.

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-ACTC1 Antikörper (ABIN112213)

Skwarek-Maruszewska, Hotulainen, Mattila, Lappalainen: "Contractility-dependent actin dynamics in cardiomyocyte sarcomeres." in: Journal of cell science, Vol. 122, Issue Pt 12, pp. 2119-26, (2009) (PubMed).

Itzhaki-Alfia, Leor, Raanani, Sternik, Spiegelstein, Netser, Holbova, Pevsner-Fischer, Lavee, Barbash: "Patient characteristics and cell source determine the number of isolated human cardiac progenitor cells." in: Circulation, Vol. 120, Issue 25, pp. 2559-66, (2009) (PubMed).

Lechler, Wu, Bernhardt, Campean, Gastiger, Hackenbeck, Klanke, Weidemann, Warnecke, Amann, Engehausen, Willam, Eckardt, Rödel, Wiesener: "The tumor gene survivin is highly expressed in adult renal tubular cells: implications for a pathophysiological role in the kidney." in: The American journal of pathology, Vol. 171, Issue 5, pp. 1483-98, (2007) (PubMed).

Details zu ACTC1

Target: ACTC1 (Actin, Alpha, Cardiac Muscle 1 (ACTC1))

Abstract: ACTC1 Produkte

Synonyme: acta1 antikoerper, actc antikoerper, fa27h01 antikoerper, fb83f06 antikoerper, wu:fa27h01 antikoerper, wu:fb83f06 antikoerper, ACTC antikoerper, ASD5 antikoerper, CMD1R antikoerper, CMH11 antikoerper, LVNC4 antikoerper, Actc-1 antikoerper, actcl antikoerper, actc1l antikoerper, cmd1r antikoerper, ACTC1 antikoerper, actin, alpha, cardiac muscle 1 L homeolog antikoerper, actin, alpha, cardiac muscle 1b antikoerper, actin, alpha, cardiac muscle 1 antikoerper, actin, alpha, cardiac muscle 1a antikoerper, actc1.L antikoerper, actc1b antikoerper, ACTC1 antikoerper, Actc1 antikoerper, actc1a antikoerper, actc1 antikoerper

Hintergrund: Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). Fetal Actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord.

Gen-ID: 70

NCBI Accession: NP_005150

UniProt: P68032

Pathways: Myometrial Relaxation and Contraction