AIF1 Antikörper (Allograft Inflammatory Factor 1)

Details for Product anti-AIF1 Antibody No. ABIN112009, Anbieter: Anmelden zum Anzeigen
Antigen
  • fa04b11
  • wu:fa04b11
  • AIF1
  • aif1
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • Bart1
  • iba1
  • mrf-1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • AIF
  • AIF1L
  • allograft inflammatory factor 1
  • Allograft inflammatory factor 1
  • allograft inflammatory factor 1-like
  • aif1
  • AIF1
  • Aif1
  • LOC100350092
  • C9ORF58
Alternativen
anti-Human AIF1 Antikörper für Immunofluorescence (fixed cells)
Reaktivität
Human, Ratte (Rattus)
206
106
96
8
7
2
2
1
1
Wirt
Maus
139
56
33
2
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser AIF1 Antikörper ist unkonjugiert
23
22
11
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
182
123
104
65
28
27
19
13
11
10
7
4
2
2
2
2
1
Optionen
Hersteller
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Immunogen Purified Human recombinant AIF-1. Epitope not further characterized.
Klon 1022-5
Isotyp IgG2b
Spezifität The antibody reacts with AIF-1.
Kreuzreaktivität (Details) Species reactivity (tested):Human and Rat.
Reinigung Affinity Chromatography.
Andere Bezeichnung AIF1 (AIF1 Antibody Abstract)
Hintergrund Allograft Inflammatory Factor-1 is a Ca2+-binding peptide produced by activated macrophages and microglial cells. It has been suggested that AIF-1 expression is associated with chronic inflammatory processes. AIF-1, like the Ca2+-binding peptides MRP8 and MRP14, is expressed by activated macrophages and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts. The recently described apoptosis-inducing factor AIF is not related to AIF-1.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1
Gen-ID 199
NCBI Accession NP_001614
UniProt P55008
Forschungsgebiet Inflammation
Pathways Smooth Muscle Cell Migration
Applikationshinweise Immunohistochemistry on Paraffin Sctions: 10-20 μg/mL (1/20-1/0). Mcrowavepretreatment for antigen retrieval recommended. Suggested positive control: Human spleen. Does not react on routinely processed Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll Immunohistochemitry With Unlabeled Monoclonal Antibodies On Paraffin-EmbeddedTissue Sections1) Deparaffinize slides in chloroform for 30min and rehydrate to distilled water2) Antigen retrieval: microwave treatment (700W) for 15min in 300ml 0. 01M citrate bufferpH 6. 0. 3) Incubate slides for 15min in 200ml methanol/4. 8ml 30%H2O24) Rinse slides 2x in distilled water and for 5min in TBS, pH 7. 55) Block with normal swine serum diluted 1: 10 in Tris buffered saline pH 7. 5, containing0. 1% bovine serum albumin (BSA). Cover sections with NSS and incubate for 15min in ahumid chamber at room temperature (RT). 6) Remove serum from sections (do not rinse)7) Incubate with primary antibody diluted in TBS/0. 1%BSA, pH 7. 5 for 60min in a humidchamber at room temperature8) Rinse sections in TBS, pH 7. 5 for 5min, 2x9) Incubate with secondary antibody (rabbit anti mouse IgG+IgM-biotin) 1: 400 diluted inTBS/0. 1%BSA, pH 7. 5 for 30min in a moist chamber, room temperature. Preincubate nowStreptABComplex /HRPO in TBS/0. 1%BSA, pH 7. 5! 10) Rinse sections in TBS, pH 7. 5 for 5min, 2x11) Incubate with StreptABComplex /HRPO for 30min in a humid chamber at roomtemperature12) Rinse sections in TBS, pH 7. 5 for 5min, 2x13) DAB-staining Prepare 3,3' - diaminobenzidintetrahydrochloride (DAB) according themanufacturers instructions (e. g. DAB tablets) and incubate slides for 1-15 min. Check colorreaction to avoid overstaining. 14) Rinse sections in TBS, pH 7. 5 for 5min, 2x15) Counterstain in haematoxylin for 1min and rinse in cold tap water for 10min16) Mount slides with coverslips
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore by adding 0.5 mL distilled water.
Konzentration 0.4 mg/mL
Buffer PBS, pH 7.2, containing 5 mg/mL BSA as stabilizer and 0.01 % Kathon as preservative.
Konservierungsmittel Kathon CG
Vorsichtsmaßnahmen This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Allgemeine Veröffentlichungen Schluesener, Seid, Meyermann: "Effects of autoantigen and dexamethasone treatment on expression of endothelial-monocyte activating polypeptide II and allograft-inflammatory factor-1 by activated macrophages and microglial cells in lesions of experimental autoimmune encephalomyelitis, n" in: Acta neuropathologica, Vol. 97, Issue 2, pp. 119-26, 1999 (PubMed).

Schluesener, Seid, Kretzschmar, Meyermann: "Allograft-inflammatory factor-1 in rat experimental autoimmune encephalomyelitis, neuritis, and uveitis: expression by activated macrophages and microglial cells." in: Glia, Vol. 24, Issue 2, pp. 244-51, 1998 (PubMed).