CD163 Antikörper

Produktnummer ABIN111878

Produktdetails anti-CD163 Antikörper

Target: CD163

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD163 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody is specific for CD163. Clone RM 3/1 recognizes a membrane glycoprotein on monocytes and macrophages which is expressed in intermediate and late inflammatory stages. Does not react with lymphocytes, granulocytes or platelets. Positive staining can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar macrophages, a major population of macrophages in the placenta, varying degrees of macrophages in inflamed tissues, including tumorous tissue depending on the inflammatory stage. Red pulp, but not white pulp macrophages of the spleen, and cortical macrophages of the thymus are detected. Macrophages in the synovialis of patients with rheumatoid arthritis. In alveolar macrophages and in Kupffer cells a double staining can be observed with clone 25F9 which is not the case in other tissues.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Monkey. Negative in Pig, Rabbit and Guinea Pig.

Aufreinigung: Affinity Chromatography

Immunogen: Human monocytes, particularly after dexamethasone treatment or after 2-5 days in culture

Klon: RM3-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 4 μg/mL (1/100). Does not work in routinely processed paraffin sections. Has been described to work in FACS and Western blots. Recommended Positive control: Human placenta.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water to a concentration of 0.4 mg/mL.

Konzentration: 0.4 mg/mL

Buffer: Stock solutions contains PBS, pH 7.2, 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted Prior to and after reconstitution at 2-8 °C. DO NOT FREEZE!

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163 (CD163 Produkte)

Synonyme: DMBT1 antikoerper, CD163 antikoerper, CD163v2 antikoerper, CD163v3 antikoerper, M130 antikoerper, MM130 antikoerper, deleted in malignant brain tumors 1 protein-like antikoerper, CD163 molecule antikoerper, CD163 antigen antikoerper, LOC426820 antikoerper, CD163 antikoerper, Cd163 antikoerper

Hintergrund: CD163 is a scavenger receptor for the haemoglobin-haptoglobin complex, and is upregulated by glucocorticoids and IL-10. The extracellular portion of the receptor is regularly shed and can be found in the circulation. An important function of CD163 seems to be in the adhesion of monocytes to activated endothelial cells. CD163-positive cells include skin histiocytes, Kupffer cells, spleen macrophages of the red pulp, and some thymus macrophages. The antigen is also found abundantly in human term placenta, and regularly in acute and chronic inflammatory lesions.Synonyms: Hemoglobin scavenger receptor, M130, Macrophage marker, Scavenger receptor cysteine-rich type 1 protein M130

Gen-ID: 9332

NCBI Accession: NP_004235

UniProt: Q86VB7