clathrin, Heavy Chain (Hc) (CLTC) Antikörper

Antigen: clathrin, Heavy Chain (Hc) (CLTC)

Synonyme: Hc, CHC, CHC17, CLH-17, CLTCL2, KIAA0034, CLTC, Chc

Klonalität: Monoklonal (CHC5-9)

Wirt: Maus

Reaktivität: Amphibien, Rind (Kuh), Human, Schwein, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Western Blot (WB)

Produktnummer: ABIN111496

Produktbeschreibung

Produktmerkmale: Synonyms: CLTC, CLH17, CLTCL2, KIAA0034, Membrane Vesicle Marker, Clathrin heavy chain onchromosome 17

Weitere Bezeichnung: Clathrin heavy chain

Gen-ID: 281080

Swiss-Prot: P49951

Immunogen: Coated vesicles (Clathrin) of Bovine brain.

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: CHC5-9

Beschreibung: Clathrin is a protein which assembles into a polyhedral network on the cell membrane asthe membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrinis composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chainswhich combine to create a distinct three-legged triskelion. It is this morphology whichallows Clathrin to form its unique polyhedral network.

Spezifität: Clone CHC5. 9 represents an excellent marker for detection of receptor mediatedendocytosis (for review cf. e. g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesiclesof various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.

Anwendungen

Applikationshinweise: Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Lagerung: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Forschungsgebiet: Organelle, Transporter

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Bruder, Wiedenmann: "Identification of a distinct 9S form of soluble clathrin in cultured cells and tissues." in: Experimental cell research, Vol. 164, Issue 2, pp. 449-62, 1986 (PubMed).

Hirst, Robinson: "Clathrin and adaptors." in: Biochimica et biophysica acta, Vol. 1404, Issue 1-2, pp. 173-93, 1998 (PubMed).

Qualmann, Kessels, Kelly: "Molecular links between endocytosis and the actin cytoskeleton." in: The Journal of cell biology, Vol. 150, Issue 5, pp. F111-6, 2000 (PubMed).