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KRT20 Antikörper

KRT20 Reaktivität: Human, Ratte, Schwein WB, IHC (p), IHC (fro), EIA Wirt: Maus Monoclonal KS20-10 unconjugated
Produktnummer ABIN111169
  • Target Alle KRT20 Antikörper anzeigen
    KRT20 (Keratin 20 (KRT20))
    Reaktivität
    • 90
    • 29
    • 26
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    Human, Ratte, Schwein
    Wirt
    • 62
    • 29
    • 3
    • 1
    • 1
    Maus
    Klonalität
    • 52
    • 44
    Monoklonal
    Konjugat
    • 62
    • 12
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dieser KRT20 Antikörper ist unkonjugiert
    Applikation
    • 67
    • 46
    • 25
    • 15
    • 13
    • 12
    • 11
    • 9
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Spezifität
    IT-Ks 20.10 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reactivities on cultured cell lines (tested so far): HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
    Aufreinigung
    Protein A affinity chromatography
    Immunogen
    Electrophoretically purified Keratin K20 from human intestinal mucosa
    Klon
    KS20-10
    Isotyp
    IgG1
    Top Product
    Discover our top product KRT20 Primärantikörper
  • Applikationshinweise
    Immunohistochemistry on frozen tissue. Immunohistochemistry on paraffin embedded sections (only after after microwavetreatment). Dilute 1/10 with PBS for immunohistochemistry. Incubation time: 1h at RT, orover night at 4C. Western blotting. ELISA.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1 mL distilled water.
    Buffer
    Final solution contains 0.09 % NaN3, 0.5 % BSA in PBS buffer, pH 7.4.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
  • de Wit, van Dalum, Lenferink, Tibbe, Hiltermann, Groen, van Rijn, Terstappen: "The detection of EpCAM(+) and EpCAM(-) circulating tumor cells." in: Scientific reports, Vol. 5, pp. 12270, (2015) (PubMed).

    Sharma, Cantz, Richter, Eckert, Henschler, Wilkens, Jochheim-Richter, Arseniev, Ott: "Human cord blood stem cells generate human cytokeratin 18-negative hepatocyte-like cells in injured mouse liver." in: The American journal of pathology, Vol. 167, Issue 2, pp. 555-64, (2005) (PubMed).

  • Target
    KRT20 (Keratin 20 (KRT20))
    Andere Bezeichnung
    Cytokeratin 20 (KRT20 Produkte)
    Synonyme
    KRT20 antikoerper, CD20 antikoerper, CK-20 antikoerper, CK20 antikoerper, K20 antikoerper, KRT21 antikoerper, Krt21 antikoerper, 9030623C06Rik antikoerper, keratin 20 antikoerper, KRT20 antikoerper, Krt20 antikoerper
    Hintergrund
    Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
    Gen-ID
    54474
    NCBI Accession
    NP_061883
    UniProt
    P35900
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