26S Proteasome (p32 subunit) Antikörper

Antigen: 26S Proteasome (p32 subunit)

Klonalität: Monoklonal (P32(26S-161))

Wirt: Maus

Reaktivität: Human, Ratte (Rattus), Xenopus laevis

Applikation: Immunfluoreszenz (IF), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN111069

Produktbeschreibung

Produktmerkmale: Synonyms: Proteasome26S

Immunogen: 26S complexes of Xenopus laevis oocytes.

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: P32(26S-161)

Spezifität: Recognizes the 20S subcomplex within the 26S heterooligomeric protein complex and thefree cytosolic form of 20S cylinder particles, the epitope is resistant to formaldehydefixation (up to 4%). Antigen Recognized in Species and Cultured Cell Lines (tested so far): Xenopus laevis,human, XLKE-A6 (Xenopus) PLC, MCF-7, A431, CaCo (human), 3T3 (mouse), RV (rat), Ptk2(rat kangaroo)

Anwendungen

Applikationshinweise: Western Blot: 1: 1000 when using the ECL method. Immunofluorescence microscopy: Ready-to-use, 20 - 30 min at RT, (longer incubation mayresult in loss of soluble antigen). P32 (26S-161) is not suitable for immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: contains 0.09% NaN3

Lagerung: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Peters: "Proteasomes: protein degradation machines of the cell." in: Trends in biochemical sciences, Vol. 19, Issue 9, pp. 377-82, 1995 (PubMed).

Peters, Franke, Kleinschmidt: "Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm." in: The Journal of biological chemistry, Vol. 269, Issue 10, pp. 7709-18, 1994 (PubMed).