GFP Antikörper (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN1107349, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
638
16
5
5
2
2
Wirt
Maus
339
171
80
40
16
9
9
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser GFP Antikörper ist unkonjugiert
33
31
28
14
12
12
12
10
9
9
8
8
7
6
6
6
6
6
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
536
354
201
156
144
134
96
87
73
25
15
13
13
8
8
6
6
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) from the Jellyfish Aequorea victoria.
Klon N-a
Isotyp IgG1
Reinigung Affinity Purified
Andere Bezeichnung GFP
Substanzklasse Tag
Hintergrund The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. When expressed in cells, fluorescence is produced without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. A number of GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wild type GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. Thus, GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.Synonyms: GFP-Tag, Green fluorescent protein
Gen-ID 6100
UniProt P42212
Forschungsgebiet Tags/Labels
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS ( pH 7.2), 0.09 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1107349) anti-Green Fluorescent Protein (GFP) antibody
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN1107349) anti-Green Fluorescent Protein (GFP) antibody (Image 2)
Allgemeine Veröffentlichungen Ouyang, Wong, Capani, Rensing, Lee, Liu, Neusch, Martone, Wu, Yamada, Ellisman, Choi: "Transient decrease in F-actin may be necessary for translocation of proteins into dendritic spines." in: The European journal of neuroscience, Vol. 22, Issue 12, pp. 2995-3005, 2005 (PubMed).

Jurado, Haserick, Lee: "Slipping or gripping? Fluorescent speckle microscopy in fish keratocytes reveals two different mechanisms for generating a retrograde flow of actin." in: Molecular biology of the cell, Vol. 16, Issue 2, pp. 507-18, 2005 (PubMed).

Wang, Bhattacharyya, Chelsea, Escobar, Banerjee: "A novel nuclear protein, MGC5306 interacts with DNA polymerase beta and has a potential role in cellular phenotype." in: Cancer research, Vol. 64, Issue 21, pp. 7673-7, 2004 (PubMed).

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