Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
This antibody detects Cyclooxygenase 1 near N-term. No cross reactivity with other proteins.
Kreuzreaktivität (Details)
Species reactivity (tested):Human, mouse, rat
Aufreinigung
Immunogen affinity purified
Immunogen
Synthetic peptide corresponding to a sequence near the N-terminal of human PTGS1(COX1) .Remarks: Sequence different to the related rat and mouse sequence by two amino acids.
Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1), is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD.By analysis of a human/hamster somatic hybrid DNA panel, Funk et al. (1991) demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91 % amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.Synonyms: COX-1, COX1, Cyclooxygenase-1, PGH synthase 1, PGHS-1, PTGS1, Prostaglandin G/H synthase 1, Prostaglandin H2 synthase 1, Prostaglandin-endoperoxide synthase 1