V5 Epitope Tag (AA 95-108) Antikörper

Details zu Produkt Nr. ABIN1104955, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
AA 95-108
14
Reaktivität
Tag
401
18
16
3
3
Wirt
Kaninchen
248
135
36
7
5
4
3
1
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
31
28
21
13
12
12
11
11
11
10
10
10
10
9
8
8
7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
366
261
137
94
77
71
51
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.

Type of Immunogen: Synthetic peptide
Sequenz GKPIPNPLLG LDST
Isotyp IgG
Spezifität This affinity-purified antibody is directed against V5 motif and is useful in determining its presence in various assays. This polyclonal anti-V5-tag antibody detects over-expressed proteins containing the V5 epitope tag. To date this antibody has reacted with all V5 tagged proteins tested so far. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. The antibody recognizes the V5-epitope tag (GKPIPNPLLGLDST) fused to either the carboxy- terminal end of targeted proteins in transfected or transformed cells. Although not yet tested, expect reactivity with recombinant proteins prepared with the V5-epitope tag fused to the amino terminal end as well.
Reinigung Affinity purified
Sterilität Sterile filtered
Andere Bezeichnung V5 Tag
Substanzklasse Tag
Forschungsgebiet Tags/Labels
Applikationshinweise Approved: ELISA, IHC (1:500 - 1:3000), WB (1:5000 - 1:10000)

Usage: Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Aliquot to Avoid freeze/thaw cycles. Store undiluted.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
Bilder des Herstellers
 image for anti-V5 Epitope Tag (AA 95-108) antibody (ABIN1104955) anti-V5 Epitope Tag (AA 95-108) antibody
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