His Tag Antikörper (HRP)

Details zu Produkt Nr. ABIN1102042, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
612
26
15
3
3
Wirt
Kaninchen
411
184
27
23
4
4
3
1
Klonalität
Polyklonal
Konjugat
HRP
57
45
36
17
14
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
549
388
164
109
78
77
74
26
13
11
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Type of Immunogen: Purified protein
Sequenz HHHHHH
Isotyp IgG
Spezifität This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Reinigung Affinity purified
Substanzklasse Tag
Forschungsgebiet Tags/Labels
Applikationshinweise Approved: ELISA, IHC (1:500 - 1:2000), WB (1:2000 - 1:5000)

Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Rekonstitution deionized water
Konzentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % Gentamicin sulfate
Konservierungsmittel Gentamicin sulfate
Vorsichtsmaßnahmen This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung avoid freeze thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Bilder des Herstellers
 image for anti-His Tag antibody (HRP) (ABIN1102042) anti-His Tag antibody (HRP)
Haben Sie etwas anderes gesucht?