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Antikörper
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anti-tyrosinase (Oculocutaneous Albinism IA) (TYR) Antikörper
tyrosinase (Oculocutaneous Albinism IA) (TYR) Antikörper
| Antigen |
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| Synonyme |
OCA1A, OCAIA, SHEP3, c, skc35, albino, sandy, zgc:109705, Tyr, C, TYR, tyr, TYRO, LOC100136546 |
| Klonalität |
Monoklonal (T311) |
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Wirt
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Reaktivität
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Applikation
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Immunfluoreszenz (IF) (21), Durchflusszytometrie (FACS) (20), Western Blot (WB) (20), Immunhistochemie (Paraffinschnitte) (IHC (p)) (17), ELISA (9), Immunhistochemie (IHC) (8), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (5), Enzyme Immunoassay (EIA) (4), Immunpräzipitation (IP) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN107987 |
| Menge |
1ml (100ug/ml approximately) (Varianten) |
| Preis |
479,81 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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Tyrosinase
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Immunogen
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BALB/C mice were injected with a recombinant tyrosinase protein.
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Format
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Supernatant
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Isotyp
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IgG2a (Passende Sekundärantikörper)
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Klon
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T311
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Spezifität
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This antibody is specific to a cluster of proteins between 70-80 kD known as tyrosinase. This antibody does not show any cross-reaction with MAGE-1 and tyrosinase-related protein-1, TRP-1/gp75. Staining of melanomas with this antibody showed tyrosinase in melanotic as well as amelanotic variants.
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Applikationshinweise
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:50 to 1:100)
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Konzentration
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100ug/ml approximately
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Alternativen zu Antigen "tyrosinase (Oculocutaneous Albinism IA) (TYR)", Typ "Antikörper" finden
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Wirte
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Kaninchen (40), Maus (12), Schaf (1)
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Reaktivitäten
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Human (52), Maus (24), Ratte (Rattus) (23), Hund (18), Rind (Kuh) (15), Schwein (15), Schaf (15), Katze (2), Fungus (1)
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Applikationen
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Immunfluoreszenz (IF) (21), Durchflusszytometrie (FACS) (20), Western Blot (WB) (20), Immunhistochemie (Paraffinschnitte) (IHC (p)) (17), ELISA (9), Immunhistochemie (IHC) (8), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (5), Enzyme Immunoassay (EIA) (4), Immunpräzipitation (IP) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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C-Term (2), AA 1-433 (1)
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Alternativen 
