Transforming Growth Factor, alpha (TGFA) Antikörper

Antigen: Transforming Growth Factor, alpha (TGFA)

Synonyme: TFGA, wa1, wa-1, TGFAA, RATTGFAA, TGFA, TGF-A, TGF alpha, LOC100009482, NV15606, DKFZp469C1513

Klonalität: Monoklonal (MF9-TG86)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN107717

Produktbeschreibung

Weitere Bezeichnung: Transforming Growth Factor-alpha (TGFa)

Immunogen: BALB/C mice were injected with synthetic peptides from rat (MF9) and human (TG86) TGF alpha.

Format: Ig-fraction

Isotyp: IgG1, IgM  (Passende Sekundärantikörper)

Klon: MF9-TG86

Spezifität: This antibody reacts with a 6 kD protein known as TGF alpha. TGF alpha is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of receptor and stimulates cell proliferation. TGF alpha plays role in tumor initiation by inducing the reversible transformed phenotype.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Anterior Pituitary. Cellular localization: Cytoplasmic

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Zytokine, Wachstumsfaktoren, Rezeptoren, Immunologie, Inflammation

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human pituitary stained with Transforming growth factor a antibody

Publikationen

Publikationen: Driman, Kobrin, Kudlow et al.: "Transforming growth factor-alpha in normal and neoplastic human endocrine tissues." in: Human pathology, Vol. 23, Issue 12, pp. 1360-5, 1993 (PubMed).