Thymidylate Synthase (TYMS) Antikörper

Antigen: Thymidylate Synthase (TYMS)

Synonyme: TS, TMS, HST422, MGC88736, Ts, MGC156654, FBgn0024920, anon-23Ca, c1.5, DmelCG3181, CG3181, zgc:56465, zgc:77659, TYMS, MGC134366, ts, tyms, MGC160415

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Rind (Kuh), Human, Maus, Ratte (Rattus), Schaf, Schwein

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN107487

Produktbeschreibung

Weitere Bezeichnung: Thymidylate Synthase

Immunogen: This whole sheep serum was prepared by repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

Format: Lyophilized

Spezifität: This antiserum is directed against human Thymidylate Synthase and is useful in determining its presence in various assays. Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining, however, some colorectal cancer specimens show very strong staining.

Anwendungen

Applikationshinweise: Note: This antiserum against Human Thymidylate Synthase (hTS) has been tested for use in ELISA, immunoprecipitation, immunofluorescence microscopy, immunohistochemistry and immunoblotting. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown. Recommended Dilutions : This product was assayed by immunoblot using HRP Rabbit-anti-Sheep IgG [H&L] (code ABIN102255) and TMB as a substrate. A working dilution of 1:1,000 to 1:5,000 is suggested for immunoblotting. A methanol based fixative can be used for immunohistochemistry using a 1:100 working dilution of the antiserum. To immunoprecipitate antigen a dilution of 1:500 is suggested. For immunoprecipitation, pre-clearing with a non-specific sheep IgG is helpful to reduce background. For ELISA a working dilution of 1:100,000 is suggested using HRP Rabbit-anti-Sheep IgG [H&L] (code ABIN102255) and ABTS as a substrate for 30 minutes at room temperature. Optimal titers for other applications should be determined by the researcher.

Konzentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, DNS/RNS, Enzyme

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (ABIN102255) diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'.

anti-Thymidylate Synthase (TYMS) antibody (Image 2)

Publikationen

Publikationen: Glaser, Staver, Waring et al.: "Gene expression profiling of multiple histone deacetylase (HDAC) inhibitors: defining a common gene set produced by HDAC inhibition in T24 and MDA carcinoma cell lines." in: Molecular cancer therapeutics, Vol. 2, Issue 2, pp. 151-63, 2003 (PubMed).

Sohn, Croxford, Yates et al.: "Effect of the methylenetetrahydrofolate reductase C677T polymorphism on chemosensitivity of colon and breast cancer cells to 5-fluorouracil and methotrexate." in: Journal of the National Cancer Institute, Vol. 96, Issue 2, pp. 134-44, 2004 (PubMed).