Replication Protein A2, 32kDa (RPA2) Antikörper

Antigen: Replication Protein A2, 32kDa (RPA2)

Synonyme: REPA2, RPA32, 153194_at, dRPA2, DmelCG9273, CG9273, rpa, zgc:109822, ik:tdsubc_2g1, xx:tdsubc_2g1, RPA2, repa2, rpa32, MGC79561, MGC137236, Rpa2, GB13976, DKFZp468O0923, LOC100230445

Klonalität: Monoklonal (9H8)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN106591

Produktbeschreibung

Weitere Bezeichnung: RPA/p34

Immunogen: BALB/C mice were injected with purified human RPA protein.

Format: Ig-fraction

Isotyp: IgG1 kappa  (Passende Sekundärantikörper)

Klon: 9H8

Spezifität: This antibody reacts with a 32-34 kD protein known as Replication Protein A (RPA). RPA is involved in DNA replication, repair and recombination. Human RPA is a stable heterotrimer of 70 kD, 32-34 kD and 11-14 kD subunits (RPA70, RPA32 and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding for SV40 DNA and stimulates DNA polymerase alpha and delta.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Immunofluorescence, Western Blotting

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: DNS/RNS

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Bilder

Formalin fixed paraffin embedded human tonsil stained with RPA/p34 antibody

Publikationen

Publikationen: Pan, Park, Amin et al.: "Phosphorylated and unphosphorylated forms of human single-stranded DNA-binding protein are equally active in simian virus 40 DNA replication and in nucleotide excision repair." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 10, pp. 4636-40, 1995 (PubMed).