Quinolinic Acid Antikörper

Antigen: Quinolinic Acid

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN106395

Produktbeschreibung

Immunogen: Synthetic Quinolinic acid conjugated to bovine serum albumin (BSA) Specificity Using a conjugate Quinolinic acid-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio(a) Quinolinic acid-BSA 1 Kynurenic-BSA 1/50,000 Phenylalanine-BSA 1/50,000 (a): Quinolinic acid-BSA concentration/Other conjugated close related compounds concentration at half displacement. BSA: Bovine Serum Albumin.

Format: Lyophilized

Isotyp: IgG  (Passende Sekundärantikörper)

Spezifität: Conjugated Quinolinic acid .

Anwendungen

Applikationshinweise: Example of Immunohistochemistry protocol Perfusion protocol for Adult male Sprague Dawley (weight around 0.5 kg):1-The animals can be deeply anaesthetized (for example with urethane-0.5-1.5g/kg, intraperitoneal). 2-Heparinized, and perfused via the ascending aorta with 50 ml of MES (2-Morpholinoethanesulfonic acid monohydrate Fluka) 10 -1 Recommended dilution for Immunohistochemistry (1/1,000-1/5,000) Recommended dilution for Western blot (1/1,000-1/12,000)

Reinheit: Antiserum previously preabsobed on protein carriers, and purified.

Lagerung: Lyophilized antibodies are stable at least 2 years. After reconstitution with 50µl of distilled water and 50µl of glycerol, the aliquot can be repeated freezed (up to five times).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Guillemin, Brew, Noonan et al.: "Indoleamine 2,3 dioxygenase and quinolinic acid immunoreactivity in Alzheimer's disease hippocampus." in: Neuropathology and applied neurobiology, Vol. 31, Issue 4, pp. 395-404, 2005 (PubMed).

Guillemin, Cullen, Lim et al.: "Characterization of the kynurenine pathway in human neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 47, pp. 12884-92, 2007 (PubMed).