Protein A Antikörper
| Antigen |
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| Klonalität |
Polyklonal |
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Wirt
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| Produktnummer |
ABIN106169 |
| Menge |
50mg (10.0 mg/ml (by UV absorbance at 280...) (Varianten) |
| Preis |
303,57 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Immunogen
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Protein A [Staphylococcus aureus]
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Format
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IgG-fraction, Lyophilized
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Beschreibung
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Anti-PROTEIN A (GOAT) Antibody
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Spezifität
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Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Goat Serum.
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Applikationshinweise
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Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. ELISA: 1:20,000 - 1:100,000. WB: 1:2,000 - 1:10,000. IHC: 1:1,000 - 1:5,000.
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Konzentration
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10.0 mg/ml (by UV absorbance at 280 nm)
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Reinheit
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This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
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Lagerung
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Store vial at 4° C prior to restoration. Restore with 5.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
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Forschungsgebiet
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Gerinnung
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Ramirez, Loch, Zhang et al.: "Use of a single chain antibody library for ovarian cancer biomarker discovery." in: Molecular & cellular proteomics : MCP, 2010 (PubMed).
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Alternativen zu Antigen "Protein A", Typ "Antikörper" finden
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Wirte
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Kaninchen (26), Ziege (25), Huhn (9)
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Reaktivitäten
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Human (8), Maus (5), Ratte (Rattus) (3), Kaninchen (1)
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Applikationen
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Western Blot (WB) (26), Immunfluoreszenz (IF) (25), Durchflusszytometrie (FACS) (23), Enzyme Immunoassay (EIA) (14), ELISA (11), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (9), Immunpräzipitation (IP) (9), Immunhistochemie (Paraffinschnitte) (IHC (p)) (5), Immunodiffusion (ID) (4), Immunhistochemie (IHC) (3), ELISA (Beschichtung) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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HRP (9), Biotin (5), FITC (4), Texas Red (TR) (2), Agarose Beads (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), DTAF (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), Rhodamine (1)
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Alternativen 
