P300/CBP Antikörper

Antigen: P300/CBP

Synonyme: CBP, KAT3A, AW558298, CBP/p300, p300/CBP, CBP_, Crbp, anon-WO0147981.11, anon-WO03040301.89, dCBP, dmCBP, nej CBP, p300, DmelCG15319, CG15319

Klonalität: Monoklonal (NM11)

Wirt: Maus

Reaktivität: Human, Affe, Nerz, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN105307

Produktbeschreibung

Immunogen: BALB/C mice were immunized with E1A affinity purified p300 protein.

Format: Purified

Isotyp: IgG2b kappa  (Passende Sekundärantikörper)

Klon: NM11

Spezifität: This antibody stains with a 300 kD protein. P300 and CBP (CREB-binding protein) are homologous to each other. P300 is transcriptional adapter protein, which is characterized by three cysteine and histidine-rich regions. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kD nuclear protein designated CBP. This antibody crossreacts with monkey, mink, mouse and rat.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified ascites containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Forschungsgebiet: Transkriptionsfaktoren

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Dallas, Yaciuk, Moran: "Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes." in: Journal of virology, Vol. 71, Issue 2, pp. 1726-31, 1997 (PubMed).

Dallas, Yaciuk, Moran: "Monoclonal antibody NM11 recognizes a C-terminal epitope shared by p300 and CBP." in: Hybridoma, Vol. 16, Issue 3, pp. 273-5, 1997 (PubMed).